Molecular and Chemical Neuropathology

, Volume 35, Issue 1–3, pp 13–21 | Cite as

Age-related changes in activity of fischer 344 rat brain acetylcholinesterase molecular forms

  • K. A. Skau
  • C. G. Triplett
Original Articles


Total acetylcholinesterase (AChE) and the molecular forms of the enzyme from six brain regions were compared in young adult (6 mo) and aged (24 mo) Fischer 344 rats. Total AChE activity was significantly reduced in aged striatum (48.7%), parietal cortex (39%), cerebellum (30.2%), and medulla/pons (23.1%). Forebrain of aged rats showed nonsignificant reduction of AChE (18.4%), but olfactory bulbs exhibited no differences in aged rats. The ratio of G4/G1 molecular forms, as isolated on sucrose density gradients, was unaltered in all aged rat brain tissues examined. These results indicate that aged rats exhibit reduced brain AChE, but there is no evidence for selective effects on individual molecular forms.

Index Entries

Acetylcholinesterase molecular forms aging rat brain Fischer 344 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Amenta F., Bronzetti E., Mancini M., Vega, J., and Zaccheo, D. (1994) Choline acetyltransferase and acetylcholinesterase in the hippocampus of aged rats: Sensitivity to choline alphoscerate treatment.Mech. Ageing Dev. 74, 47–58.PubMedCrossRefGoogle Scholar
  2. Bisso, G., Briancesco R., and Michalek, H. (1991) Size and charge isomers of acetylcholinesterase in the cerebral cortex of young and aged rats.Neurochem. Res. 16, 571–575.PubMedCrossRefGoogle Scholar
  3. Ellman G. L., Courtney K.D., Andres V., and Featherstone R.M. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity.Biochem. Pharmacol. 7, 88–95.PubMedCrossRefGoogle Scholar
  4. Geula C., and Mesulam, M. (1989) Special properties of cholinesterases in the cerebral cortex of Alzheimer’s disease.Brain Res. 498, 185–189.PubMedCrossRefGoogle Scholar
  5. Glowinski J., and Iversen, L.I. (1966) Regional studies of catecholamines in the rat brain.J. Neurochem 13, 655–669.PubMedCrossRefGoogle Scholar
  6. Gorini A., Ghigini B., and Villa R. (1996) Acetylcholinesterase activity of synaptic plasma membranes during ageing: Effect ofl-acetylcarnitine.Dementia 7, 147–154.PubMedCrossRefGoogle Scholar
  7. Hammond P. and Brimijoin S. (1988) Acetylcholinesterase in Huntington’s and Alzheimer’s diseases: Simultaneous enzyme, assay and immunoassay of multiple brain regions.J. Neurochem. 50, 1111–1116.PubMedCrossRefGoogle Scholar
  8. James T., and Kanungo M. (1976) Alterations in atropine sites of the brain of rats as a function of age.Biochem. Biophys. Res. Commun. 72, 170–175.PubMedCrossRefGoogle Scholar
  9. Lai J., Leung T., and Lim L. (1981) Brain regional distribution of glutamic acid decarboxylase, choline acetyltransferase, and acetylcholinesterase in the rat: Effects of chronic manganese chloride administration after two years.J. Neurochem. 36, 1443–1448.PubMedCrossRefGoogle Scholar
  10. Massoulié J., and Bon S. (1982) The molecular forms of cholinesterase and acetylcholinesterase in vertebrates.Annu. Rev. Neurosci. 5, 57–106.PubMedCrossRefGoogle Scholar
  11. Massoulié J., Pezzementi L., Bon S., Krejci E., and Vallette F. (1993) Molecular and cellular biology of cholinesterases.Prog. Neurobiol. 41, 31–91.PubMedCrossRefGoogle Scholar
  12. Meneguz A., Bisso G., and Michalek H. (1992) Age-related changes in acetylcholinesterase and its molecular forms in various brain areas of rats.Neurochem. Res. 17, 785–790.PubMedCrossRefGoogle Scholar
  13. Mesulam M., and Moran M. (1987) Cholinesterases within neurofibrillary tangles related to age and Alzheimer’s Disease.Ann. Neurol. 22, 223–228.PubMedCrossRefGoogle Scholar
  14. Michalek H., Fortuna S., and Pintor A. (1989) Age-related differences in, brain choline acetyltransferase, cholinesterases and muscarinic receptor sites in two strains of rats.Neurobiol. Aging 10, 143–148.PubMedCrossRefGoogle Scholar
  15. Morin A., and Wasterlain C. (1980) Aging and rat brain muscarinic receptors as measured by quinuclidinyl benzilate binding.Neurochem. Res. 5, 301–308.PubMedCrossRefGoogle Scholar
  16. Pintor A., Fortuna S., Volpe M., and Michalek, H. (1988) Muscarinic receptor plasticity in the brain of senescent rats: Down-regulation after repeated administration of diisopropyl fluorphosphate.Life Sci. 42, 2113–2121.PubMedCrossRefGoogle Scholar
  17. Santucci A., Kanof P., and Haroutunian V. (1993) Cholinergic marker deficits induced by lesions of the nucleus basalis of meynert are attenuated by nerve growth factor in young, but not in aged, F344 rats.Brain Res. 609, 327–332.PubMedCrossRefGoogle Scholar
  18. Sastry B., Janson V., Jaiswal N., and Tayeb O. (1983) Changes in enzymes of the cholinergic system and acetylcholine release in the cerebra of aging male Fischer rats.Pharmacology 26, 61–72.PubMedCrossRefGoogle Scholar
  19. Siek G., Katz L., Fishman E., Korosi T., and Marquis J. (1990) Molecular forms of acetylcholinesterase in subcortical areas of normal and Alzheimer Disease brain.Biol. Psychiatry 27, 573–580.PubMedCrossRefGoogle Scholar
  20. Silman I., Lyles J., and Barnard E. (1978) Intrinsic forms of acetylcholinesterase in skeletal muscle.FEBS Lett. 94, 166–170.PubMedCrossRefGoogle Scholar
  21. Sirvio J., Valjakka A., Jolkkonen J., Hervonen A., and Riekkinen P. (1988) Cholinergic enzyme activities and muscarinic binding in the cerebral cortex of rats of different age and sex.Comp. Biochem. Physiol. 90C, 245–248.Google Scholar
  22. Sirvio J., Riekkinen P., and Hervonen A. (1989) Age-dependence of the solubility fractions of acetylcholinesterase in the cerebral cortex and cerebellum of the rat.Neurosci. Lett. 96, 218–222.PubMedCrossRefGoogle Scholar
  23. Skau K. (1985) Acetylcholinesterase molecular forms in serum and erythrocytes of laboratory animals.Comp. Biochem. Physiol. 80C, 207–210.Google Scholar
  24. Skau K., and Brimijoin S. (1981) Abnormal distribution of skeletal muscle acetylcholinesterase molecular forms in dystrophic mice.Exp. Neurol. 74, 111–121.PubMedCrossRefGoogle Scholar
  25. Sketelj J., Crne-Finderle J., and Brzin M. (1992) Influence of denervation on the molecular forms of junctional and extrajunctional acetylcholinesterase in fast and slow muscles of the rat.Neurochem. Int. 21, 415–421.PubMedCrossRefGoogle Scholar
  26. Sketelj J., Leisner E., Gohlsch B., Skorjanc D., and Pette D. (1997) Specific impulse patterns regulate acetylcholinesterase activity in skeletal muscles of rat and rabbit.J. Neurosci. Res. 47, 49–57.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  1. 1.Division of Pharmaceutical SciencesUniversity of Cincinnati College of PharmacyCincinnati

Personalised recommendations