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Characterization of the limited specificity of antigen recognition in earthworms

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Abstract

Earthworms respond to parenteral stimulation with a protein antigen by the formation of an antigen-binding protein. Earthworms were parenterally stimulated with different proteins and the protein binding was estimatedin vitro on both humoral and cellular levels. The binding was significantly higher when the same protein was used forin vivo stimulation. The degree of specificity of the antigen-binding protein after the secondaryin vivo challenge increased, but even so it was considerably lower than that of vertebrate immunoglobulins.

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Abbreviations

ABP:

antigen-binding protein

LBSS:

Lumbricus balanced salt solution

ARS-HSA:

arsanylated human serum albumin

BSA:

bovine serum albumin

LPS:

lipopolysaccharide

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Dedicated to Professor J. Šterzl on the occasion of his 70th birthday

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Bilej, M., Tučková, L. & Romanovský, A. Characterization of the limited specificity of antigen recognition in earthworms. Folia Microbiol 40, 436–440 (1995). https://doi.org/10.1007/BF02814753

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