Maintenance ofRhodotorula rubra isolated from liquid samples of gold mine effluents
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TheRhodotorula rubra strain isolated from waste waters of a gold mining plant has demonstrated the ability to grow in the presence of cyanide. The maintenance of this strain in complex organic media leads to a loss of this ability. To preserve the cyanide resistance ofR. rubra we tested the following maintenance methods: subculturing in sterile distilled water, freezing at −20, −40, −70°C, liquid nitrogen freezing and the paper replica method. The ability to grow in the presence of cyanide was preserved and a higher viability level was observed for cells maintained frozen at −70°C, in liquid nitrogen and by the paper replica method. Preservation in distilled water resulted in the lowest viability after twelve months of storage.
KeywordsCyanide Sole Nitrogen Source Basal Salt Medium Sabouraud Agar Brewing Yeast
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- Kirsop B.E.: Maintenance of yeasts, pp. 109–189 in B.E. Kirsop, J.S. Snell:A Manual of Laboratory Methods. Academic Press, London 1984.Google Scholar
- Odds F.C.: UK Federation for Culture Collections Newsletter, pp. 6–7 (1976).Google Scholar
- Silva L.F., Kamita N.F., Oliveira M.S., Altherthum F.: Freeze-drying of industrial yeast strains: Influence of growth conditions, cooling rates and suspending media on the viability of recovered cells.Rev. Microbiol.23, 177–182 (1992).Google Scholar