Folia Microbiologica

, Volume 40, Issue 5, pp 487–489 | Cite as

Maintenance ofRhodotorula rubra isolated from liquid samples of gold mine effluents

  • V. R. Linardi
  • M. C. Amancio
  • N. C. M. Gomes


TheRhodotorula rubra strain isolated from waste waters of a gold mining plant has demonstrated the ability to grow in the presence of cyanide. The maintenance of this strain in complex organic media leads to a loss of this ability. To preserve the cyanide resistance ofR. rubra we tested the following maintenance methods: subculturing in sterile distilled water, freezing at −20, −40, −70°C, liquid nitrogen freezing and the paper replica method. The ability to grow in the presence of cyanide was preserved and a higher viability level was observed for cells maintained frozen at −70°C, in liquid nitrogen and by the paper replica method. Preservation in distilled water resulted in the lowest viability after twelve months of storage.


Cyanide Sole Nitrogen Source Basal Salt Medium Sabouraud Agar Brewing Yeast 
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Copyright information

© Folia Microbiologica 1995

Authors and Affiliations

  • V. R. Linardi
    • 1
  • M. C. Amancio
    • 1
  • N. C. M. Gomes
    • 1
  1. 1.Departmento de Microbiologia, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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