Folia Microbiologica

, Volume 39, Issue 3, pp 208–214 | Cite as

Optimization of rhizobialnod gene-inducing activity assay in pea root exudate

  • K. Novák
  • V. Škrdleta
  • M. Němcová
  • L. Lisá


ThelacZ transcriptional fusion assay of the activation of rhizobialnod genes has been adapted for the microtitration plates. The assay can be performed reproducibly on a microscale, including induction, lysis, and spectrophotometry. The technique facilitates processing of a large number of samples in physiological studies. Using the microplate method, the time course of the release ofnod-gene inducers from the root of noninoculated, hydroponically cultivated, pea (Pisum sativum L.) plants was monitored till the 16th day after germination. The released activity increased with plant age but a high variability among individual plants and days was observed.


Flavonoid Quercetin Root Exudate Naringenin Suspension Density 
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sodium dodecyl sulfate


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Copyright information

© Folia Microbiologica 1994

Authors and Affiliations

  • K. Novák
    • 1
  • V. Škrdleta
    • 1
  • M. Němcová
    • 1
  • L. Lisá
    • 1
  1. 1.Laboratory of Biological Nitrogen Fixation, Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague 4Czech Republic

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