Folia Microbiologica

, Volume 39, Issue 3, pp 171–175 | Cite as

Construction of recombinant K88 DNA with Ptac promoter

  • E. Holoda
  • I. Mikula


The gene encoding K88ab was localized on the 11.6 kbHindIII-HindIII fragment of 74 kb plasmid DNA ofE. coli 7301. The smallest recombinant DNA producing the K88ab antigen was obtained by excision of the 5.15 kbEcoRI-EcoRI fragment from recombinant DNA composed of the 11.6 kb K88ab fragment in the pBR322 vectro. The size of the smallest fragment was 6.5 kb. Expression of the K88ab antigen was controlled by the P1 promoter of the pBR322 vector. Substitution of promoter Ptac for promoter P1 made it possible to achieve expression of the K88ab antigen byE. coli MT. Substitution of promoter PL for promoter P1 failed to achieve expression of the K88 ab antigen in the recipient strains used.


Transformation Product Klenow Fragment Chymosin Thermal Induction pBR322 Vector 
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© Folia Microbiologica 1994

Authors and Affiliations

  • E. Holoda
    • 1
  • I. Mikula
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of Veterinary MedicineKošiceSlovak Republic

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