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Folia Microbiologica

, Volume 41, Issue 3, pp 237–242 | Cite as

Three fluorescent probes for the flow-cytometric assessment of membrane potential inSaccharomyces cerevisiœ

  • B. Denksteinová
  • K. Sigler
  • J. Plášek
Papers

Abstract

Three fluorescent probes, tetramethyl rhodamine ethyl ester (TMRE), 3,3′-dipropylthiacarbocyanine iodide (diS-C3(3)) and 3,3′-dipropyloxacarbocyanine iodide (diO-C3(3)), were tested for their suitability as fluorescent indicators of membrane potential inSaccharomyces cerevisiœ in studies performed by flow cytometry. For all these dyes the intensity of fluorescence of stained cells increased with probe concentration in the range of 60–3000 nmol/L. The optimum staining period was 15–20 min for diS-C3(3). Depolarization of cells by increased extracellular potassium level and by valinomycin elicited with all probes a drop in fluorescence intensity. In some yeast batches this depolarization was accompanied by a separation of subpopulations with different fluorescence properties.

Keywords

Fluorescence Intensity Membrane Potential Valinomycin Probe Concentration Killer Toxin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Folia Microbiologica 1996

Authors and Affiliations

  • B. Denksteinová
    • 1
  • K. Sigler
    • 2
  • J. Plášek
    • 1
  1. 1.Institute of PhysicsCharles UniversityPrague 2Czech Republic
  2. 2.Institute of MicrobiologyCzech Academy of SciencesPrague 4Czech Republic

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