Folia Microbiologica

, Volume 37, Issue 1, pp 17–23 | Cite as

Cell growth and α-amylase production characteristics ofBacillus subtilis

  • K. Dercová
  • J. Augustín
  • D. Krajčová


Growth, differential rate of α-amylase synthesis and production characteristics ofBacillus subtilis DP 1 (isolate from starch materials) in comparison with 10Bacillus strains were examined in batch fermentation. The effect of the carbon and nitrogen source was evaluated with regard to cell growth and enzyme production. The pH optimum of enzyme activity was 6.5 and temperature optimum 60°C.


Shake Flask Amylase Activity Soluble Starch Bacillus Strain Bacillus Amyloliquefaciens 
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  1. Alam S., Hong J., Weigand W.A.: Effect of yeast extract on α-amylase synthesis byBacillus amyloliquefaciens.Biotechnol. Bioeng.33, 780–785 (1989).CrossRefPubMedGoogle Scholar
  2. Bajpai P., Bajpai P.K.: High temperature alkaline α-amylase fromBacillus licheniformis TCRDC-B13.Biotechnol. Bioeng.33, 72–78 (1989).CrossRefPubMedGoogle Scholar
  3. Bernfeld P.: Amylases α and β, pp. 149–158 inMethods in Enzymology, Vol. 1 (N. Kaplan, Ed.), Academic Press, New York 1955.CrossRefGoogle Scholar
  4. De Mot R., Andries K., Verachtert H.: Comparative study of starch degradation and amylase production by ascomyceteous yeast species.System. Appl. Microb.5, 106–118 (1984).Google Scholar
  5. Liebs P., Riedel K., Graba J.P., Schrapel D., Tischler U.: Formation of some extracellular enzymes during the exponential growth ofBacillus subtilis.Folia Microbiol.33, 88–95 (1988).CrossRefGoogle Scholar
  6. Priest F.G.: Extracellular enzyme synthesis in the genusBacillus.Bacteriol. Rev.41, 711–753 (1977).PubMedGoogle Scholar
  7. Takehito Y.: Data on individual amylases, pp. 18–125 inHandbook of Amylases and Related Enzymes (The Amylase Research Society of Japan, Ed.), Pergamon Press, Oxford 1988.Google Scholar
  8. Toda K.: Induction and repression of enzymes in microbial culture.J. Chem. Technol. Biotechnol.31, 775–790 (1981).CrossRefGoogle Scholar
  9. Yoo Y.J., Cadman T.W., Hong J., Hatch R.T.: Kinetics of α-amylase synthesis fromBacillus amyloliquefaciens Biotechnol. Bioeng.31, 357–365 (1988).CrossRefPubMedGoogle Scholar
  10. Yoon M.Y., Yoo Y.J., Cadman T.W.: Phosphate effects in the fermentation of α-amylase byBacillus amyloliquefaciens.Biotech. Lett.11, 57–60 (1989).CrossRefGoogle Scholar

Copyright information

© Folia Microbiologica 1992

Authors and Affiliations

  • K. Dercová
    • 1
  • J. Augustín
    • 1
  • D. Krajčová
    • 1
  1. 1.Department of Biochemical Technology, Faculty of Chemical TechnologySlovak Technical UniversityBratislavaCzechoslovakia

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