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Folia Microbiologica

, Volume 38, Issue 1, pp 49–54 | Cite as

Effect of lipids and detergents on the production and solubilization of extracellular lipase inYarrowia lipolytica

  • Č. Novotnyý
  • l. Doležalová
Papers

Abstract

Triglycerides, oleic acid but not fatty acid-containing, nonionic detergents (Spans, Tweens) were able to stimulate the synthesis of cell-bound and soluble lipase ofYarrowia lipolytica grown in a complex medium containing citrate and urea. The optimal concentration of olive oil for induction was 0.5% (W/V). The combined effect of a high ionic strength (0.75 mol/L KCl) and of digitonin (2 mmol/L) at pH 7.6 resulted in solubilization of 80% of the cell-bound lipase and a significant activation of the enzyme. Comparison of twoY. lipolytica strains showed the effects of Mg2+ and Fe3+ concentrations in the medium on the synthesis of the enzyme to be strongly strain-dependent.

Keywords

Lipase Lipase Activity High Ionic Strength Lipase Production Digitonin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Folia Microbiologica 1993

Authors and Affiliations

  • Č. Novotnyý
    • 1
  • l. Doležalová
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague 4

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