Folia Microbiologica

, Volume 38, Issue 1, pp 49–54 | Cite as

Effect of lipids and detergents on the production and solubilization of extracellular lipase inYarrowia lipolytica

  • Č. Novotnyý
  • l. Doležalová


Triglycerides, oleic acid but not fatty acid-containing, nonionic detergents (Spans, Tweens) were able to stimulate the synthesis of cell-bound and soluble lipase ofYarrowia lipolytica grown in a complex medium containing citrate and urea. The optimal concentration of olive oil for induction was 0.5% (W/V). The combined effect of a high ionic strength (0.75 mol/L KCl) and of digitonin (2 mmol/L) at pH 7.6 resulted in solubilization of 80% of the cell-bound lipase and a significant activation of the enzyme. Comparison of twoY. lipolytica strains showed the effects of Mg2+ and Fe3+ concentrations in the medium on the synthesis of the enzyme to be strongly strain-dependent.


Lipase Lipase Activity High Ionic Strength Lipase Production Digitonin 


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Copyright information

© Folia Microbiologica 1993

Authors and Affiliations

  • Č. Novotnyý
    • 1
  • l. Doležalová
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague 4

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