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l-phenylalanine production by double auxotrophic multianalogue-resistant mutant ofArthrobacter globiformis

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Abstract

Tryptophan-plus-tyrosine double auxotrophic mutants resistant to fluorophenylalanine (PFP) and β-2-thienylalanine (TA) were isolated from a biotin-requiring glutamate-producingArthrobacter globiformis. The mutants were found to producel-phenylalanine in mineral salts medium. Further improvement ofl-phenylalanine production was achieved by isolation of mutants resistant to 5-methyltryptophan (MT) and 3-nitrotyrosine (NT) from a double auxotrophic PFPr and TAr mutant. Under optimal cultural condition one mutant yielded 9.6g phenylalanine per L medium in flask culture. Enzymic activity of regulatory enzymes (deoxy-d-arabino-heptulosonate-7-phosphate synthase, chorismate mutase and prephenate dehydratase) were observed in the wild type, double auxotroph and double-auxotrophic multianalogue-resistant mutant.

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  1. Microbiology Laboratory, Department of Botany, Burdwan University, 713 104, Burdwan, West Bengal, India

    T. K. Maiti & S. P. Chatterjee

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  1. T. K. Maiti
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  2. S. P. Chatterjee
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Maiti, T.K., Chatterjee, S.P. l-phenylalanine production by double auxotrophic multianalogue-resistant mutant ofArthrobacter globiformis . Folia Microbiol 39, 387–391 (1994). https://doi.org/10.1007/BF02814444

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