Skip to main content
SpringerLink
Log in

Production ofl-phenylalanine by double auxotrophic mutants ofArthrobacter globiformis: Effect of temperature, trace salts and inoculum dose

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Three tryptophan-plus-tyrosine double auxotrophic mutants were isolated from a biotin-requiring glutamate-producingArthrobacter globiformis. The mutants were found to producel-phenylalanine in a mineral salt medium. Further improvement ofl-phenylalanine production was achieved by isolation of mutants resistant to β-2-thienylalanine from these double auxotrophs. Temperature of 30 °C and a 4% inoculum dose were found to be optimum for phenylalanine production. Addition of some trace salts does not enhance phenylalanine yield. Under optimal cultural conditions one mutant yielded 6.8 g phenylalanine per L medium in flask culture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akashi K., Shibai H., Hirose Y.: Effect of O2 supply onl-phenylalanine,l-proline,l-glutamine andl-arginine fermentation.J. Ferment. Technol. 57, 331–327 (1979).

    Google Scholar 

  • Alforldi L.: La production induite de megacine en milieu synthetique.Ann. Inst. Pasteur 94, 474–485 (1958).

    Google Scholar 

  • Banerjee A.K., Nandi P.: Effect of trace elements on the growth and antibiotic production byStreptomyces griseus strain AC4248.Trans. Bose Res. Inst. 27, 87–92 (1964).

    Google Scholar 

  • de Boer L., Dijkhuizen L.: Microbial and enzymatic process forl-phenylalanine production.Adv. Biochem. Bioeng/Biotechnol. 41, 1–27 (1990).

    Google Scholar 

  • Calhown D.H., Jensen R.A.: Significance of altered carbon flow in aromatic amino acid synthesis on approach to the isolation of regulatory mutants ofPseudomonas aeruginosa.J. Bacteriol. 55, 466–475 (1972).

    Google Scholar 

  • Chao K., Foster J.W.: A glutamic acid producingBacillus sp.J. Bacteriol. 77, 715–721 (1959).

    PubMed  CAS  Google Scholar 

  • Chattopadhyay S.P., Banerjee A.K.: Production ofl-glutamic acid by aBacillus sp.Folia Microbiol. 23, 469–480 (1978).

    CAS  Google Scholar 

  • Choi Y.J., Tribe D.E.: Continuous production of phenylalanine using onE. coli regulatory mutant.Biotechnol. Lett. 4, 223–228 (1982).

    Article  CAS  Google Scholar 

  • Coats J.H., Nester E.W.: Regulation reversal mutation characterisation of end product activated mutants ofBacillus subtilis.J. Biol. Chem. 242, 4948–4955 (1967).

    PubMed  CAS  Google Scholar 

  • Crosby G.A.: New swetners.CRC Crit. Food Sci. 7, 297–323 (1976).

    CAS  Google Scholar 

  • Hagino H., Nakayama K.:l-Phenylalanine production by analogue resistant mutant ofCorynebacterium glutamicum.Agr. Biol. Chem. 38, 157–161 (1974).

    CAS  Google Scholar 

  • Hwang S.O., Gil G.H., Cho Y.J., Kang K.R., Lee J.H., Bae J.C.: The fermentation process forl-phenylalanine production using an auxotrophic regulatory mutant ofE. coli.Appl. Microbiol. Biotechnol. 22, 108–113 (1985).

    Article  CAS  Google Scholar 

  • Klausner A.: Building for success in phenylalanine.Biotechnology 3, 301–307 (1985).

    Article  Google Scholar 

  • Maiti T.K., Chatterjee S.P.: Microbial production ofl-phenylalanine: A review.Hind. Ant. Bull. 32, 3–26 (1990).

    CAS  Google Scholar 

  • Maiti T.K., Chatterjee S.P.:l-Phenylalanine production by double auxotrophic mutant ofArthrobacter globiformis.Folia Microbiol. 36, 234–239 (1991a).

    Article  CAS  Google Scholar 

  • Maiti T.K., Chatterjee S.P.: Production ofl-phenylalanine by double auxotrophic mutant ofArthrobacter globiformis: Optimization of C and N source.Acta Biotechnol. 11, 249–254 (1991b).

    Article  CAS  Google Scholar 

  • Okumura S., Otsuka S., Yamanoi A., Yoshinaga F., Honda T., Kubota K., Tsuchida T.:l-Phenylalanine.US Pat. 3 600 235 (1972).

  • Roy D.K., Chatterjee S.P.: Production of glutamic acid by anArthrobacter sp. I. Nutritional requirement in relation to glutamic acid production.Acta Microbiol. Polon. 3, 117–122 (1982).

    Google Scholar 

  • Sen K., Chatterjee S.P.: Extracellular lysine production from hydrocarbon byArthrobacter globiformis.Folia Microbiol. 28, 292–300 (1983).

    CAS  Google Scholar 

  • Sen S.K., Chatterjee S.P.: Influence of antibiotics and trace salts on lysine production byArthrobacter globiformis.Acta Biotechnol. 5, 215–219 (1985).

    Article  CAS  Google Scholar 

  • Shetty K., Crawford D.L., Pometto IIIA.L.: Production ofl-phenylalanine from starch by analogue resistant mutants ofBacillus polymyxa.Appl. Environ. Microbiol. 52, 637–643 (1986).

    PubMed  CAS  Google Scholar 

  • Suzuki M., Berglund A., Unden A., Heden C.G.: Aromatic amino acid production by analogue resistant mutants ofMethylomonas methanophila 6R.J. Ferment. Technol. 55, 466–475 (1977).

    CAS  Google Scholar 

  • Tokoro Y., Oshima K., Okii M., Yamaguchi K., Tanaka K., Kinoshita S.: Microbial production ofl-phenylalanine fromn-alkanes.Agr. Biol. Chem. 34, 1516–1521 (1970).

    CAS  Google Scholar 

  • Tsuchida T., Kubota K., Morinaga Y., Matsui H., Enei H., Yoshinaga F.: Production ofl-phenylalanine byBrevibacterium lactofermentum 2256.Agr. Biol. Chem. 51, 2055–2101 (1987).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Microbiology Laboratory, Department of Botany, Burdwan University, 713 104, Burdwan, West Bengal, India

    T. K. Maiti

Authors
  1. T. K. Maiti
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Maiti, T.K. Production ofl-phenylalanine by double auxotrophic mutants ofArthrobacter globiformis: Effect of temperature, trace salts and inoculum dose. Folia Microbiol 38, 447–450 (1993). https://doi.org/10.1007/BF02814393

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02814393

Keywords

Search

Navigation