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Folia Microbiologica

, Volume 36, Issue 3, pp 234–239 | Cite as

l-Phenylalanine production by double auxotrophic mutants ofArthrobacter globiformis

  • T. K. Maiti
  • S. P. Chatterjee
Papers

Abstract

A number of tryptophan-plus-tyrosine double auxotrophs have been isolated from a glutamate producingArthrobacter globiformis excretingl-phenylalanine by two-step mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine. For the three potent mutants tested the medium of Alföldi was found to be the best. The optimum tryptophan, tyrosine and biotin concentrations for phenylalanine production of these mutants were 0.5 mmol/L, 0.1 mmol/L and 5 μg/L, respectively. At these levels strain TT-39 yielded 2.6 g phenylalanine per L of medium in flask culture with glucose (350 mmol/L) and NH4Cl (60 mmol/L).

Keywords

Mineral Salt Medium Corynebacterium Glutamicum Arthrobacter Globiformis Biotin Concentration Prephenate Dehydrogenase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1991

Authors and Affiliations

  • T. K. Maiti
    • 1
  • S. P. Chatterjee
    • 1
  1. 1.Microbiology Laboratory, Department of BotanyBurdwan UniversityBurdwanIndia

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