Folia Microbiologica

, Volume 40, Issue 3, pp 267–270 | Cite as

Effect of soluble maillard reaction products on rumen microorganisms

  • M. Marounek
  • V. A. Kostyukovsky
  • B. Hodrová
  • K. Fliegerová


After incubation of Maillard reaction polymers (MRPs) with rumen fluid from wethers neither volatile fatty acids nor lactate were produced. Soluble polymeric products of the Maillard reaction were nonmetabolizable by a mixed culture of rumen microorganisms. MRPs added at 0.5 and 2 g/L inhibited the growth of seven ruminal Gram-negative bacteria by 20 and 30%, respectively. In four strains of Gram-positive bacteria, MRPs lowered the cell concentration by 11% (0.5 g/L) and 25% (2 g/L). The rumen fungusOrpinomyces jojonii also did not metabolize soluble MRPs.


Volatile Fatty Acid Maillard Reaction Rumen Fluid Rumen Bacterium Rumen Microorganism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Association of Official Analytical Chemists:Official Methods of Analysis. Washington (DC) 1980.Google Scholar
  2. Brock T.D., O'Dea K.: Amorphous ferrous sulphide as a reducing agent for culture of anaerobes.Appl. Environ. Microbiol. 33, 254–256 (1977).PubMedGoogle Scholar
  3. Caldwell D.R., Bryant M.P.: Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria.Appl. Microbiol. 14, 794–801 (1966).PubMedGoogle Scholar
  4. Conway E.J.:Microdiffusion Analysis and Volumetric Error, 4th ed.. Crosby Lockwood & Son, London 1957.Google Scholar
  5. Dennison C., Marais J.P.: The influence of ruminant salivary buffer salts upon thein vitro microbial digestion of forages.Agroanimalia 12, 1–6 (1980).Google Scholar
  6. Einarsson H.: The effect of time, temperature, pH and reactants on the formation of antibacterial compounds in the Maillard reaction.Lebensm. Wiss. Technol. 20, 51–55 (1987).Google Scholar
  7. Einarsson H., Snygg B.G., Eriksson C.: Inhibition of bacterial growth by Maillard reaction products.J. Agric. Food Chem. 31, 1043–1047 (1983).CrossRefGoogle Scholar
  8. Einarsson H., Eklund T., Nes I.F.: Inhibitory mechanisms of Maillard reaction products.Microbios 53, 27–36 (1988).PubMedGoogle Scholar
  9. Herbert D., Phipps P.J., Strange R.E.: Chemical analysis of microbial cells, pp. 272–277 in J.R. Norris, D.W. Ribbons (Eds):Methods in Microbiology, Vol. 5B. Academic Press, London 1971.Google Scholar
  10. Hillebrant W.G., Lundell G.E.F., Bright H.A., Hoffman J.I.:Applied Inorganic Analysis. SNTL, Prague 1958.Google Scholar
  11. Marounek M., Březina P.: Heat-induced formation of soluble Maillard reaction products and its influence on utilization of glucose by rumen bacteria.Arch. Anim. Nutr. Berlin 43, 45–51 (1993).CrossRefGoogle Scholar
  12. McWeeny D.J., Knowles M.E., Hearne J.F.: The chemistry of non-enzymic browning in foods and its control by sulphites.J. Sci. Food Agric. 25, 735–746 (1974).CrossRefGoogle Scholar
  13. Ottenstein D.M., Bartley D.A.: Improved gas chromatography separation of free acids C2−C5 in dilute solutionAnal. Chem. 43, 952–955 (1971).CrossRefGoogle Scholar
  14. Sleat R., Mah R.A.: Quantitative methods for colorimetric determination of formate in fermentation media.Appl. Environ. Microbiol. 47, 884–885 (1984).PubMedGoogle Scholar
  15. Sniffen C.J., O'Connor J.D., van Soest P.J., Fox D.G., Russell J.B.: A net carbohydrate and protein system for evaluating cattle diets. II. Carbohydrate and protein availability.J. Anim. Sci. 70, 3562–3577 (1992).PubMedGoogle Scholar
  16. van Soest P.J.:Nutritional Ecology of the Ruminant. O. & B Books Inc.. Corvallis (USA) 1983.Google Scholar
  17. van Soest P.J., Mason V.C.: The influence of the Maillard reaction upon the nutritive value of fibrous feeds.Anim. Feed Sci. Technol. 32, 45–53 (1991).CrossRefGoogle Scholar

Copyright information

© Folia Microbiologica 1995

Authors and Affiliations

  • M. Marounek
    • 1
  • V. A. Kostyukovsky
    • 1
  • B. Hodrová
    • 1
  • K. Fliegerová
    • 1
  1. 1.Institute of Animal Physiology and GeneticsAcademy of Sciences of the Czech RepublicPrague 10

Personalised recommendations