A plant-based expression system for matching cDNA clones and isozymes
Isozymes of barley α-amylase were matched to cDNAs that encode them using transient expression in oat aleurone layers. Four cDNAs, including two that are previously unpublished, were inserted into oat aleurone cells by microparticle bombardment. The cDNAs were under the control of theAct1 promoter of rice. Expression levels were sufficient for in-gel detection of enzyme activity following isoelectric focusing of aleurone homogenates. The system has also proved useful in characterizing a hybrid β-glucanase gene.
Key WordsAvena sativa α-amylase cDNAs cDNA isozyme matching Hordeum vulgare isoelectric focusing microparticle bombardment oat aleurone transient expression
rice actin gene
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