In Vitro

, Volume 12, Issue 6, pp 460–466 | Cite as

Comparative study of extracellular amino acids in culture of human liver and fibroblastic cells

  • F. Lemonnier
  • M. Gautier
  • N. Moatti
  • A. Lemonnier


Amino acid concentrations are studied in the extracellular media of ten series of human fibroblast and liver cell monolayer cultures. These two cell types consume and produce ostensively the same amino acids. Among the nonessential amino acids, the most significant variations involve serine and aspartate which are decreased; α-alanine, glutamate, ornithine and proline are, on the contrary, increased. Among the essential amino acids, leucine, isoleucine and glutamine are preferentially decreased. The variations of some amino acids are correlated with the cell density. The interrelations which may exist between the variations of these different amino acids are discussed. Furthermore, the glycolytic activity of the cells studied is very high: 85% of glucose consumed is found in the form of lactate.

Key words

amino acids culture human liver cells human fibroblasts glycolysis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Taylor, P. E., A. J. Zuckerman, and L. J. Farrow. 1969. Culture of needle biopsies of the liver from patients with suspected hepatitis. J. Clin. Pathol. 22: 701–703.PubMedCrossRefGoogle Scholar
  2. 2.
    Le Guilly, Y., P. Lenoir, M. Bourel, R. Poupon, and A. Guillouzo. 1970. La culture prolongée du tissu hépatique humain adulte. Pathol. Biol. 18: 733–741.PubMedGoogle Scholar
  3. 3.
    Rioche, M., S. Quelin, I. Seck, M. Jacquesson, B. Basteris, and R. Masseyef. 1970. Culturein vitro de cellules de cancer primitif de foie humain. C. R. Acad. Sci. Paris 271: 1148–1151.Google Scholar
  4. 4.
    Demoise, C. F., J. T. Galambos, and A. Falek. 1971. Tissue culture of adult human liver. Gastroenterology 60: 390–399.PubMedGoogle Scholar
  5. 5.
    Okigaki, T., and A. L. Koehler. 1972. Characteristics of human liver cells in culture. 1972. J. Cell. Biol. 55: 193a.Google Scholar
  6. 6.
    Hsia, D. Y. Y. 1970. Study of hereditary metabolic diseases usingin vitro techniques. Metabolism 19: 309–339.PubMedCrossRefGoogle Scholar
  7. 7.
    Milunsky, A., J. W. Littlefield, J. N. Kanfer, E. H. Kolodny, V. E. Shih, and L. Atkins. 1970. Prenatal genetic diagnosis. New Engl. J. Med. 283: 1370–1381.PubMedCrossRefGoogle Scholar
  8. 8.
    Schulman, J. D., and K. H. Bradley. 1971. Cystinosis therapeutic implications ofin vitro studies of cultured fibroblasts. J. Pediatr. 78: 833–836.PubMedCrossRefGoogle Scholar
  9. 9.
    Kroll W. A., F. L. A. Becker, and J. A. Schneider. 1974. Measurement of intracellular amino acids in cultured skin fibroblasts. Biochem. Med. 10: 368–373.PubMedCrossRefGoogle Scholar
  10. 10.
    Fischer, A. 1948. Amino acid metabolism of tissue cellsin vitro. Biochem. J. 43: 491–497.PubMedGoogle Scholar
  11. 11.
    Piez, K., and H. Eagle. 1958. The free amino acid pool of cultured human cells. J. Biol. Chem. 231: 533–545.PubMedGoogle Scholar
  12. 12.
    Eagle, H. 1959. Amino acid metabolism in mammalian cell cultures. Science 130: 432–437.PubMedCrossRefGoogle Scholar
  13. 13.
    Eagle, H., and L. Levintow. 1965. Amino acid and protein metabolism. Chapter VIII. In: E. N. Willmer (Ed.),Cells and Tissues in Culture Methods. Biology and Physiology I. Academic Press, New York, pp. 277–296.Google Scholar
  14. 14.
    Lucy, J. A. 1965. Amino acid and protein metabolism. Chapter IX. In: E. N. Willmer (Ed.),Cells and Tissues in Culture Methods. Biology and Physiology I. Academic Press, New York, pp. 297–314.Google Scholar
  15. 15.
    Paul, J. 1972. Behaviour of cells in culture: growth, differentiation and metabolism.Cell and Tissue Culture. 4th edition. Churchill Livingstone, Edinburgh and London, pp. 25–51.Google Scholar
  16. 16.
    Melancon, S. B., J. Tayco, and H. L. Nadler. 1972. The free amino acid pool of cultivated human skin fibroblasts (36783). Proc. Soc. Exp. Biol. Med. 141: 391–395.PubMedGoogle Scholar
  17. 17.
    McCarty, K. 1962. Selective utilization of amino acids by mammalian cell cultures. Exp. Cell Res. 27: 230–240.CrossRefGoogle Scholar
  18. 18.
    Griffiths, J. B. 1970. The quantitative utilization of amino acids and glucose and contact inhibition of growth in cultures of the human diploid cell W1 38. J. Cell. Sci. 6: 739–749.PubMedGoogle Scholar
  19. 19.
    Stoner, G. D., and D. J. Merchant. 1972. Amino acid utilization by L. M. strain mouse cells in a chemically defined medium. In Vitro 7: 330–343.PubMedGoogle Scholar
  20. 20.
    Hayflick, L., and P. S. Moorhead. 1961. The serial cultivation of human diploid cell strain. Exp. Cell. Res. 25: 585–621.CrossRefGoogle Scholar
  21. 21.
    Lemonnier, F., M. L. Cornec, M. Golovtchenko, and M. Gautier. 1974. Synthèse de l’albumine au cours de cultures issues de parenchume hépatique d’origine humaine. Biochimie 56: 1111–1117.PubMedCrossRefGoogle Scholar
  22. 22.
    Markert, C. L. 1957. In: Some trends in research on cellular differentiation and morphogenesis. J. Natl. Cancer Inst. 19: 632.Google Scholar
  23. 23.
    Smith, P. F. 1960. Amino acid metabolism of P.P.L.O. Ann. N.Y. Acad. Sci. 79: 543–550.PubMedCrossRefGoogle Scholar
  24. 24.
    Westfall, B. B., E. V. Peppers, and W. R. Earle. 1955. The change in concentration of certain constituents of the medium during growth of the strain Hela cells. Am. J. Hyg. 61: 326–333.PubMedGoogle Scholar
  25. 25.
    Griffiths, J. B. 1972. Role of serum, insulin and amino acid concentration in contact of growth of human cells in culture. Exp. Cell Res. 75: 47–56.PubMedCrossRefGoogle Scholar
  26. 26.
    Litwin, J. 1972. Human diploid cell response to variations in relative amino acid concentrations in Eagle medium. Exp. Cell Res. 72: 566–568.PubMedCrossRefGoogle Scholar
  27. 27.
    Litwin, J. 1974. Growth of human diploid fibroblasts in media with different amino acid composition. J. Cell Sci. 14: 671–680.PubMedGoogle Scholar
  28. 28.
    Griffiths, J. B. 1972. The effect of cell population density on nutrient uptake and cell metabolism: a comparative study of human diploid and heteroploid cell lines. J. Cell Sci. 10: 515–524.PubMedGoogle Scholar
  29. 29.
    Meisler, A. I. 1973. Studies on contact inhibition of growth in the mouse fibroblast 3T3. II. Effects of amino acid deprivation and serum on growth rate. J. Cell Sci. 12: 861–873.PubMedGoogle Scholar

Copyright information

© Tissue Culture Association 1976

Authors and Affiliations

  • F. Lemonnier
    • 1
  • M. Gautier
    • 1
  • N. Moatti
    • 1
  • A. Lemonnier
    • 1
  1. 1.Centre Hospitalier de BicêtreKremlin-BicêtreFrance

Personalised recommendations