Comparative study of extracellular amino acids in culture of human liver and fibroblastic cells
- 26 Downloads
Amino acid concentrations are studied in the extracellular media of ten series of human fibroblast and liver cell monolayer cultures. These two cell types consume and produce ostensively the same amino acids. Among the nonessential amino acids, the most significant variations involve serine and aspartate which are decreased; α-alanine, glutamate, ornithine and proline are, on the contrary, increased. Among the essential amino acids, leucine, isoleucine and glutamine are preferentially decreased. The variations of some amino acids are correlated with the cell density. The interrelations which may exist between the variations of these different amino acids are discussed. Furthermore, the glycolytic activity of the cells studied is very high: 85% of glucose consumed is found in the form of lactate.
Key wordsamino acids culture human liver cells human fibroblasts glycolysis
Unable to display preview. Download preview PDF.
- 3.Rioche, M., S. Quelin, I. Seck, M. Jacquesson, B. Basteris, and R. Masseyef. 1970. Culturein vitro de cellules de cancer primitif de foie humain. C. R. Acad. Sci. Paris 271: 1148–1151.Google Scholar
- 5.Okigaki, T., and A. L. Koehler. 1972. Characteristics of human liver cells in culture. 1972. J. Cell. Biol. 55: 193a.Google Scholar
- 13.Eagle, H., and L. Levintow. 1965. Amino acid and protein metabolism. Chapter VIII. In: E. N. Willmer (Ed.),Cells and Tissues in Culture Methods. Biology and Physiology I. Academic Press, New York, pp. 277–296.Google Scholar
- 14.Lucy, J. A. 1965. Amino acid and protein metabolism. Chapter IX. In: E. N. Willmer (Ed.),Cells and Tissues in Culture Methods. Biology and Physiology I. Academic Press, New York, pp. 297–314.Google Scholar
- 15.Paul, J. 1972. Behaviour of cells in culture: growth, differentiation and metabolism.Cell and Tissue Culture. 4th edition. Churchill Livingstone, Edinburgh and London, pp. 25–51.Google Scholar
- 22.Markert, C. L. 1957. In: Some trends in research on cellular differentiation and morphogenesis. J. Natl. Cancer Inst. 19: 632.Google Scholar