In Vitro

, Volume 12, Issue 6, pp 436–441 | Cite as

Occurrence of minute ring-shaped nucleoprotein particles in culture media conditioned by mammalian cells

  • K. Shankar Narayan
  • Neima Hendren


Conditioned media of 21 mammalian cell lines have been examined by electron microscopic and biochemical techniques for the presence of a minute ring-shaped nucleoprotein particle (RSP). Electron microscopy showed the presence of RSP in 20 of the 21 cell lines tested. Comparative analyses of six cell cultures for radio-active RSP, following3H-thymidine incorporation, suggested that quantitative differences exist in the amount of RSP detectable in conditioned media of normal and pathologic cells. The cell lines tested included cells of different morphologies, origin and function.

Key words

minute ring-shaped nucleoprotein particles cultured cells 


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Copyright information

© Tissue Culture Association 1976

Authors and Affiliations

  • K. Shankar Narayan
    • 1
  • Neima Hendren
    • 1
  1. 1.Pasadena Foundation for Medical ResearchPasadena

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