Plant Molecular Biology Reporter

, Volume 20, Issue 1, pp 59–65 | Cite as

The dried corncob as a source of DNA for PCR analysis

  • M. C. Schneerman
  • J. Mwangi
  • B. Hobart
  • J. Arbuckle
  • D. A. Vaske
  • J. C. Register
  • D. F. Weber


We developed a procedure to isolate DNA from dried corncobs. This DNA was amplified successfully by PCR, producing well-defined bands in response to specific primers. The SSR patterns between cob and leaf DNA of the same inbred line were found to be identical, indicating that the DNA in the cob cells had not degraded during senescence.

Key words

corncob DNA PCR 


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Copyright information

© Springer 2002

Authors and Affiliations

  • M. C. Schneerman
    • 1
  • J. Mwangi
    • 2
  • B. Hobart
    • 3
  • J. Arbuckle
    • 4
  • D. A. Vaske
    • 3
  • J. C. Register
    • 3
  • D. F. Weber
    • 2
  1. 1.Department of Crop SciencesUniversity of IllinoisUrbana
  2. 2.Illinois State UniversityNormalDepartment of Biological Sciences
  3. 3.Pioneer Hi-Bred International, Inc.Johnston
  4. 4.SygentaStanton

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