International Journal of Pancreatology

, Volume 1, Issue 5–6, pp 389–398 | Cite as

Rat exocrine pancreas following total gastrectomy

  • Markus Büchler
  • Peter Malfertheiner
  • Bernd Glasbrenner
  • Hans G. Beger
Research Papers


Sixteen rats underwent a total gastrectomy with an end-to-end anastomosis between the esophagus and duodenum. After either two or four weeks, the entire pancreas was excised and isolated lobules were incubated for 60 min in Krebs-Ringer-Hepes solution either with or without addition of ceruletide (5 × 10-10 g/ml). Amylase was determined every 15 min in the supernatant medium. Measurements of amylase, lipase, trypsin, DNA and total protein were carried out in the homogenized pancreatic tissue. Morphometry was performed via light microscopic analysis. Two weeks following gastrectomy there was an increase in pancreatic wet weight, DNA, total protein and enzyme content (except lipase). Four weeks after surgery, pancreatic weight, amylase and trypsin increased further, whereas DNA content and lipase content remained stable. The DNA concentration decreased, and the size of exocrine pancreatic cells increased. In vitro, amylase discharge increased only after four weeks. The experiments demonstrate a short-term adaptation of the rat exocrine pancreas following total gastrectomy in the form of cellular hyperplasia (after 2 weeks), and hypertrophy (after 4 weeks), and enzyme dissociation.

Key words

Exocrine pancreatic charges rat total gastrectomy 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Deveney, C.W., Owen, R.L., Deveney, K., Reber, H.A. and Way, L.W., Effect of acid secretory capacity and chronic endogenous hypergastrinaemia on pancreatic secretion and intestinal morphology in the rat, Dig. Dis. Sci., 1983; 28: 65–73.PubMedCrossRefGoogle Scholar
  2. 2.
    Debas, H.T. and Yamagishi, T., Evidence for pyloropancreatic reflex for pancreatic exocrine secretion, Am. J. Physiol., 1978; 234: 468–471.Google Scholar
  3. 3.
    Johansson, H., Grimelius, L., Heitz, P.U., Lundquist, G., Peterson, P., Portela-Gomez, G. and Wilander, E., The effects of endogenous hypergastrinemia and hypogastrinemia on the exocrine and endocrine rat pancreas, Uppsala J. Med. Sci., 1979; 84: 247–254.Google Scholar
  4. 4.
    Stock-Damgé, C., Aprahamian, M., Lhoste, E., Pousse, A., Humbert, W., Nariega, R. and Grenier, J.F., Pancreatic hyperplasia after small bowel resection in the rat: dissociation from endogenous gastrin levels, Digestion, 1984; 29: 223–230.PubMedCrossRefGoogle Scholar
  5. 5.
    Johnson, L.R., The trophic action of gastrointestinal hormones, Gastroenterology, 1976; 70: 278–288.PubMedGoogle Scholar
  6. 6.
    Dembinski, A.B. and Johnson, L.R., Growth of pancreas and gastrointestinal mucosa in antrectomized and gastrin-treated rats, Endocrinology, 1979; 105: 769–773.PubMedCrossRefGoogle Scholar
  7. 7.
    White, T.T., Lundh, G. and Magee, D.F., Evidence for the existence of a gastropancreatic reflex, Am. J. Physiol., 1960; 198: 725–728.PubMedGoogle Scholar
  8. 8.
    Malfertheiner, P., Junge, U. and Ditschuneit, H., Pancreatic digestive function after subtotal gastrectomy, Hepatogastroenterology, 1984; 31: 172–175.PubMedGoogle Scholar
  9. 9.
    Gullo, L., Costa, P.L., Ventrucci, M., Mattioli, S., Viti, G. and Labo, G., Exocrine pancreatic function after total gastrectomy. Scand. J. Gastroenterol., 1979; 14: 401–407.PubMedGoogle Scholar
  10. 10.
    MacGregor, I., Parent, J. and Meyer, J.H., Gastric emptying of liquid meals and pancreatic and biliary secretion after subtotal gastrectomy or truncal vagotomy and pyloroplasty in man, Gastroenterology, 1977; 72: 195–205.PubMedGoogle Scholar
  11. 11.
    Scheele, G.A. and Palade, G.E., Studies on the guinea pig pancreas, J. Biol. Chem., 1975; 250: 2660–2670.PubMedGoogle Scholar
  12. 12.
    Schick, H.J., Regulation der Proteinbiosynthese im exokrinen Pankreas der Ratte unter verschiedenen Bedingungen, Inaugural Dissertation, University of Marburg, F.R.G., 1982.Google Scholar
  13. 13.
    Bernfeld, P., Amylases, α+ Β. In: Methods in Enzymology, Vol. 1. Academic Press, New York, 1955: 149–158.CrossRefGoogle Scholar
  14. 14.
    Hummel, B.W.C., A modified spectrophotometric determination of chymotrypsin, trypsin and thrombin, Can. J. Biochem. Biophys., 1959; 37: 1393–1399.Google Scholar
  15. 15.
    Verduin, C.A., Pant, R. and Kreuter, H.H., Studies on the determination of lipase activity, Clin. Chim. Acta, 1973:46: 11–19.PubMedCrossRefGoogle Scholar
  16. 16.
    Lowry, O. H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., Protein measurement with the folin phenol reagent, J. Biol. Chem., 1951; 193: 265–275.PubMedGoogle Scholar
  17. 17.
    Richards, G.M., Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA, Anal. Biochem., 1974; 57: 369–376.PubMedCrossRefGoogle Scholar
  18. 18.
    Maystor, P.P. and Barrowman, J.A., The influence of chronic administration of pentagastrin on the rat pancreas, Q.J. Exp. Physiol., 1971; 56: 113–122.Google Scholar
  19. 19.
    Büchler, M., Malfertheiner, P., Griener, B., Fischbach, W., Ditschuneit, H. and Beger, H.G., The influence of large bowel on pancreatic enzyme secretion and content, Digestion, 1984; 30: 88–89.Google Scholar
  20. 20.
    Büchler, M., Malfertheiner, P., Block, S., Griener, L.B., Fischbach, W. and Beger, H.G., The influence of subtotal small bowel resection on pancreatic enzyme secretion and content, Dig. Dis. Sci., 1984; 29: 944–945.Google Scholar
  21. 21.
    Malfertheiner, P., Büchler, M., Griener, B. and Ditschuneit, H., Segmental bowel resection its specific influence on the exocrine pancreas in rats. Eur. J. Clin. Invest., 1985; 15: 122–123.Google Scholar
  22. 22.
    Bloom, S.R. and Polak, J.M., Enteropancreatic axis. In: Bloom, S.R. (Ed.), Gut Hormones, Churchill Livingston, Edinburgh and London, 1978; 151–163.Google Scholar
  23. 23.
    Singer, M.V., Untersuchungen zur hormonalen und nervalen Regulation der Pankreassekretion., Fortschr. Med., 1981; 99: 272–276.PubMedGoogle Scholar
  24. 24.
    Lin, T.M., Evans, C., Shaar, C.J. and Root, M.A., Action of somatostatin on stomach, pancreas gastric mucosal blood flow and hormones. Am. J. Physiol., 1983; 244: 40–45.Google Scholar
  25. 25.
    Schusdziarra, V., Rouiller, D., Pietri, A., Harris, V., Zysnar, E., Carlon, J.M. and Unger, R.H., Pancreatic and gastric release of somatostatin-like immunoreactivity during intestinal phase of a meal. Am. J. Physiol., 1979; 237: 555–560.Google Scholar
  26. 26.
    Modlin, I.M., Jaffe, B.M., Albert, D., Materia, A., Crochelt, R. and Sank, A., The role of the antrum in the modulation of plasma pancreatic polypeptide levels, J. Surg. Res., 1981; 30: 269–274.PubMedCrossRefGoogle Scholar
  27. 27.
    Fölsch, U.R., Winckler, K. and Wormsley, K.G., Influence of repeated administration of CCK and secretin on the pancreas of the rat, Scand. J. Gastroenterol., 1978; 13: 663–671.PubMedCrossRefGoogle Scholar
  28. 28.
    Schneemann, B.O., Chang, J., Smith, J.R. and Lyman, R.L., Effect of dietary amino acids, casein and soybean trypsin inhibitor on pancreatic protein secretion in rats, J. Nutr., 1977; 107: 281–288.Google Scholar

Copyright information

© Elsevier Science Publishers B.V. (Biomedical Division) 1986

Authors and Affiliations

  • Markus Büchler
    • 1
  • Peter Malfertheiner
    • 2
  • Bernd Glasbrenner
    • 1
  • Hans G. Beger
    • 1
  1. 1.Department of SurgeryUniversity of UlmUlmFRG
  2. 2.Department of GastroenterologyUniversity of UlmUlmFRG

Personalised recommendations