Summary
Chymotrypsin, trypsin, carboxypeptidase A and B, elastase and enterokinase activities were measured in buffer solutions and in human duodenal juice after incubation with wheat bran, cellulose, guar gum, pectin, psyllium and lignin. The different types of dietary fiber led to inhibition of enzymatic activity in most experiments, e.g., lignin could totally ablish the activity of isolated trypsin and chymotrypsin. Only in enterokinase was there no influence. Inhibition depended on incubation time; the effect was proportional to fiber concentration and inversely related to enzyme level. Treatment of fiber with hydrochloric acid (pH 1.5) and heat (95‡C) destroyed inhibitory activity in some experiments. The effect of lignin on one enzyme (trypsin) was reduced by the addition of another enzyme (chymotrypsin). It is concluded that dietary fiber could affect digestion by inhibiting proteolytic pancreatic enzymes.
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Isaksson, G. and Ihse, I. Effects of dietary fiber on pancreatic enzyme activities of ileostomy evacuates and on excretion of fat and nitrogen in the rat, Scand. J. Gastroenterol., 1983; 18: 417–423.
Shah, N., Atallah, M.T., Mahoney, R.R. and Pellett, P.L. Effect of dietary fiber components on fecal nitrogen excretion and protein utilization in growing rats, J. Nutr., 1982; 112: 658–666.
Schneider, M.U., Domschke, S., Heptner, G. and Domschke, W. Einflu\ von Pflanzenfasern auf die lipolytische und proteolytische exokrine Pankreasfunktion, Dtsch. Med. Wschr., 1984; 109: 250–253.
Smail Poksay, K. and Schneeman, B.O. Pancreatic and intestinal response to dietary guar gum in rats, J. Nutr., 1983; 113: 1544–1549.
Isaksson, G., Lilja, P., Lundquist, I. and Ihse, I. Influence of dietary fiber on exocrine pancreatic function, Digestion, 1983; 27: 57–62.
Stock-Damgé, C., Bouchet, P., Dentinger, A., Aprahamian, M. and Grenier, J.F. Effect of dietary fiber supplementation on the secretory function of the exocrine pancreas in the dog, Am. J. Clin. Nutr., 1983; 38: 843–848.
Sommer, H. and Kasper, H. The effect of dietary fiber on the pancreatic excretory function, Hepato-Gastroenterology, 1980; 27: 477–483.
Elsenhans, B., Sufke, U., Blume, R. and Caspary, W.F. The influence of carbohydrate gelling agents on rat intestinal transport of monosaccharide and neutral amino acids in vitro, Clin. Sci., 1980; 59: 373–380.
Dunaif, G. and Schneeman, B.O. The effect of dietary fiber on human pancreatic enzyme activity in vitro, Am. J. Clin. Nutr., 1981; 34: 1034–1035.
Isaksson, G., Lundquist, I. and Ihse, I. In vitro inhibition of pancreatic enzyme activities by dietary fiber, Digestion, 1982; 24: 54–59.
Dutta, S.K. and Hlasko, J. Dietary fiber in pancreatic disease: effect of high fiber diet on fat malabsorption in pancreatic insufficiency and in vitro study of the interaction of dietary fiber with pancreatic enzymes, Am. J. Clin. Nutr., 1985; 41: 517–525.
Erlanger, B.F., Kokowsky, W. and Cohen, W. The preparation and properties of two new chromogenic substrates of trypsin, Arch. Biochem., 1961; 95: 271–278.
Nagel, W., Willig, F., Peschke, W. and Schmidt, F.H. über die Bestimmung von Trypsin und Chymotrypsin mit AminosÄure-p-nitroaniliden, Hoppe-Seylers Z. Physiol. Chem., 1965; 340: 1–10.
Appel, W. Carboxypeptidasen. In: H.U. Bergmeyer (Ed.), Methoden der Enzymatischen Analyse, 2nd ed., Verlag Chemie, Weinheim, 1970: 906–961.
Appel, W. Ekstase. In: H.U. Bergmeyer (Ed.), Methoden der Enzymatischen Analyse, 2nd ed., Verlag Chemie, Weinheim, 1970: 993–997.
Niessen, K.H., Schmidt, K. and Brügmann, G. SekundÄrer Enterokinase-Mangel der Duodenalschleimhaut bei partieller und totaler Zottenatrophie. Mschr. Kinderheilk., 1973; 121: 49–53.
Hansen, W.E. and Schulz, G. The effect of dietary fiber on pancreatic amylase activity in vitro, Hepato-Gastroenterology, 1982; 29: 157–160.
Isaksson, G., Lundquist, I. and Ihse, I. Effect of dietary fiber on pancreatic enzyme activity in vitro. The importance of viscosity, pH, ionic strength, adsorption, and time of incubation, Gastroenterology, 1982; 82: 918–924.
Brown, W. Interactions of small molecules with hydrated polymer networks. In: G.E. Inglett and S.I. Falkehag (Eds.), Dietary Fibers: Chemistry and Nutrition, Academic Press, New York, 1979: 1–13.
Hansen, W.E., Schulz, G. Lösliche und fixierte Inhibitoren der Amylase. In: K. Huth and C. BrÄuning (Eds.), Pflanzenfasern—Neue Wege in der Stoffwechseltherapie, Karger, Basel, 1983: 144–149.
Tanford, C. Physical chemistry of macromolecules, Wiley, New York, 1961: 587–668.
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Hansen, W.E. Effect of dietary fiber on proteolytic pancreatic enzymes in vitro. Int J Pancreatol 1, 341–351 (1986). https://doi.org/10.1007/BF02801866
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DOI: https://doi.org/10.1007/BF02801866