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Basic studies of hydrogen evolution by escherichia coli containing a cloned citrobacter freundii hydrogenase gene

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Abstract

Citrobacter freundii genes that complementedEscherichia coli hyd(hydrogenase activity) mutation were cloned in plasmids pCBH4 (6.2 kb) and pCBH6(5.7 kb) (1,2). Hydrogen evolution by the transformantE. coli HK-8(pCBH4 or pCBH6) was investigated. The optimum culture temperature of recombinantE. coli cells for hydrogen evolution from glucose was in the neighborhood of 18°C. The recombinantE. coli cells cultured at this condition showed a several-fold increase of hydrogen evolution, as compared with that of the wild-type cells. The plasmid-retention stability of this recombinantE. coli was extremely high, especially plasmid pCBH4, which was completely retained during 2 wk without any restriction. Hydrogen production by immobilized recombinantE. coli was then investigated using cells cultured at 18°C. The hydrogen evolution rate from glucose and Lennoxbroth were about twofold higher than that ofE. coli C600, and this high hydrogen evolution rate was maintained for more than 1 mo.

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Authors and Affiliations

  1. Tokyo Research Laboratory, Japan Synthetic Rubber Co., Ltd., Higashi-Yurigaoka 3-5-1, 215, Asao-ku Kawasaki, Japan

    Hlsamori Kanayama

  2. Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsuta-cho Midori-ku, 227, Yokohama, Japan

    Koji Sode & Isao Karube

Authors
  1. Hlsamori Kanayama
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  2. Koji Sode
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  3. Isao Karube
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Kanayama, H., Sode, K. & Karube, I. Basic studies of hydrogen evolution by escherichia coli containing a cloned citrobacter freundii hydrogenase gene. Appl Biochem Biotechnol 15, 97–106 (1987). https://doi.org/10.1007/BF02801311

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  • DOI: https://doi.org/10.1007/BF02801311

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