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Plant Molecular Biology Reporter

, Volume 20, Issue 2, pp 189–189 | Cite as

An expeditious method for constructing T-vectors usingEam 1105 I cassettes

  • Hu Xuejun
  • Zhang Zhichao
  • Bao Yongming
  • Yang Qing
  • An Lijia
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Abstract

An expeditious method is described for constructing T-vectors containing complementary 3′-thymidine overhangs. A T-vector was developed by cloning a 90-bpEam 1105 I cassette containing 2Eam 1105 I restriction sites into a modified pUC119 vector. TheEam 1105 I cassette was generated by PCR with 2 specific primers containing different recognition sequences ofEam 1105 I. The recombinant vector was easily converted into a T-vector by digestion of the plasmid withEam 1105 I. The cloning efficiency of the PCR product was approximately 90%. The method described here is a simple way to construct a variety of T-vectors.

Key words

Eam 1105 I cassette PCR products T-vector 

Copyright information

© International Society for Plant Molecular Biology 2002

Authors and Affiliations

  • Hu Xuejun
    • 1
  • Zhang Zhichao
    • 1
  • Bao Yongming
    • 1
  • Yang Qing
    • 1
  • An Lijia
    • 1
  1. 1.Department of BioengineeringDalian University of TechnologyDalianChina

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