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Soft-laser scanning densitometry performed on a 35-mm slide illustrating SDS-PAGE separation of adenovirion polypeptides

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Abstract

Virion polypeptides (35 S), methionine-labeled and purified by CsCl gradient centrifugation, were separated by SDS-gel electrophoresis. Analysis of their band pattern was performed by scanning the images of the SDS-gels shown on a 35-mm slide. The densitometric tracings revealed the presence of 17 protein bands, although only 15 of them were visible to the naked eye. The high sensitivity and resolving capacities of the soft-laser scanning densitometer enabled us to detect trace amounts of protein bands separated in SDS-gels and to obtain a resolution compatible to that of electrophoresis. Fourfold electronic amplification of the densitometric tracings, produced by a computer, generated new information regarding the pattern of the electrophoregram. The facility to amplify peaks of importance is particularly advantageous when faint or overlapping protein bands revealed on a gel are assessed.

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References

  1. Anderson, G. N., Anderson, L. N., and Tollaksen, L. S. (1979),Clin. Chem. 25,(7) 1199.

    CAS  Google Scholar 

  2. Diezel, W., Kopperschlager, G., and Hoffman, E. (1972),Anal. Biochem. 48, 617.

    Article  CAS  Google Scholar 

  3. Weber, J., Begin, M., and Carstens, E. B. (1977),Virology 76, 709.

    Article  CAS  Google Scholar 

  4. Scheff, G., Marrow, C., Pillay, K. G., and Zeineh, R. A. (1974),Clin. Res. 22(3) 434A.

    Google Scholar 

  5. Zeineh, R. A., Mbawa, E. H., Pillay, V. G., Fiorella, B., and Dunea, G. (1973),J. Lab. Clin. Med. 82, 326.

    CAS  Google Scholar 

  6. Zeineh, R. A., and Nijm, W. P. (1974),Clin. Res. 22(3) 428A.

    Google Scholar 

  7. Zeineh, R. A., Nijm, P. W., and Al-Azzawi, H. F. (1975),Am. Lab. 7(2) 51.

    CAS  Google Scholar 

  8. Porro, M., Viti, S., Antoni, G., and Saletti, M. (1982),Anal. Biochem. 127, 316.

    Article  CAS  Google Scholar 

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Zeineh, R.A., Kyriakidis, G. & Bhatti, A.R. Soft-laser scanning densitometry performed on a 35-mm slide illustrating SDS-PAGE separation of adenovirion polypeptides. Appl Biochem Biotechnol 13, 111–117 (1986). https://doi.org/10.1007/BF02798904

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  • DOI: https://doi.org/10.1007/BF02798904

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