Abstract
Growth and lactic acid production ofLactobacillus delbreuckii were compared using glucose and lactose as carbon sources. A continuous-flow stirred-tank fermenter was coupled with a cross-flow filtration unit to permit operation at high-cell concentrations. At steady state, yeast extract requirements for lactic-acid production were lower when glucose was used as a substrate than with lactose fermentation. Once steady state was obtained, with glucose feed, it was possible to lower the yeast extract concentration without affecting biomass concentration and lactic acid production. The lacticacid concentration that inhibited cell growth and lactic acid production was found to depend on the choice of a carbon substrate.
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References
Cysewski, G. R., and Wilke, C. R. (1977),Biotechnol. Bioeng. 19 1125.
Lee, K.J., Lefebre, M., Tribe, D. E., and Rogers, P. L. (1980),Biotechnol. Lett. 2, 487.
Cheryan, M., and Mehaia, M. W. (1984),Biotechnol. Lett. 5 519.
Ohleyer, E., Blanch, H. W., and Wilke, C. R.Appl. Biochem. Biotechnol. (to appear).
Kirk-Othmer (1981), in Encyclopedia of Chemical Technology, vol. 13, 3rd ed., Wiley, NY.
Vick Roy, T. B. (1985), inComprehensive Biotechnology, vol. III, Pergamon, UK.
Maiorella, B. L., and Castillo, F. J. (1984),Process Biochem. 19 157.
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Ohleyer, E., Wilke, C.R. & Blanch, H.W. Continuous production of lactic acid from glucose and lactose in a cell-recycle reactor. Appl Biochem Biotechnol 11, 457–463 (1985). https://doi.org/10.1007/BF02798641
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DOI: https://doi.org/10.1007/BF02798641