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Immunocapillarymigration

Use of fluorescein- and enzyme-labeled antibodies to quantify C-reactive protein

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Abstract

The method is based upon the attachment of antibodies to a porous insoluble support and the subsequent capillary migration of the antigen-containing solution in the support. The antigen-covered area thereby obtained was visualized with fluoresceinor horseradish peroxidase-labeled antibodies. The height of this area increased with increasing antigen concentration. The method was used to quantitate C-reactive protein.

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Glad, C. Immunocapillarymigration. Appl Biochem Biotechnol 7, 75–79 (1982). https://doi.org/10.1007/BF02798627

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  • DOI: https://doi.org/10.1007/BF02798627

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