Abstract
In a competitive binding assay, the ligand to be quantified competes with a fixed amount of labeled ligand for the sites on a limiting amount of binding protein. The amount of label bound is therefore dependent on the ratio between native and labeled ligand. In a binding assay, one must separate the free ligands from bound. The better the separation, the higher the sensitivity of the assay. But effective methods are often laborious and time-consuming and thus we have developed a novel approach, the Partition Affinity Ligand Assay (PALA).
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References
Mattiasson, B., and Ling, T. G. I.,J. Immunol. Meth. 38, 217 (1980).
Mattiasson, B.,J. Immunol. Meth. 35, 137 (1980).
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Ling, T.G.I., Mattiasson, B. Partition Affinity Ligand Assay (PALA). Appl Biochem Biotechnol 7, 71–74 (1982). https://doi.org/10.1007/BF02798626
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DOI: https://doi.org/10.1007/BF02798626