Abstract
The present report describes a new approach in soft laser scanning densitometry performed on opaque electrophoregrams. The method employs a light reflectance system instead of absorption, which is commonly used. The data of this preliminary study were obtained by directly scanning a photograph of an electrophoregram exhibiting viral proteins separated by SDS-PAGE. Comparison of the two methods clearly demonstrate that laser reflectance offers finer resolution than laser absorbance when opaque materials are scanned.
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Zeineh, R.A., Kyriakidis, G. Use of reflectance in soft laser densitometry in scanning opaque electrophoregrams. Appl Biochem Biotechnol 12, 25–30 (1986). https://doi.org/10.1007/BF02798576
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DOI: https://doi.org/10.1007/BF02798576