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Determination of the polyamine content of rat heart mitochondria by the use of heparin-sepharose

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Abstract

Heparin-sepharose has been utilized to remove polyamines adsorbed to the cytoplasmic surface of rat heart mitochondria. The results obtained can be summarized as follows:

  1. 1.

    Heparin-sepharose removes 90% of the spermine, 98% of the spermidine, and 98% of the putrescine adsorbed.

  2. 2.

    Polyamine contents of chromatographed mitochondria amount to 2.66 and 0.36 nmol spermine and spermidine, respectively, per mg of mitochondrial protein.

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Authors and Affiliations

  1. Istituto di Chimica Biologica, Università di Bologna, Via Irnerio 48, 40126, Bologna, Italy

    B. Tadolini, L. Cabrini, G. Piccinini, P. P. Davalli & A. M. Sechi

  2. Istituto di Chimica Biologica, Università di Modena, Via Campi 287, 41100, Modena, Italy

    G. Piccinini

  3. Istituto di Chimica Biologica, Università di Parma, Via Gramsci14, 43100, Parma, Italy

    P. P. Davalli

Authors
  1. B. Tadolini
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  2. L. Cabrini
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  3. G. Piccinini
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  4. P. P. Davalli
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  5. A. M. Sechi
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Tadolini, B., Cabrini, L., Piccinini, G. et al. Determination of the polyamine content of rat heart mitochondria by the use of heparin-sepharose. Appl Biochem Biotechnol 11, 173–176 (1985). https://doi.org/10.1007/BF02798473

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  • DOI: https://doi.org/10.1007/BF02798473

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