Abstract
For the continuous production ofl-aspartic acid from fumaric acid and ammonia by the action of aspartase, the enzyme extracted fromEscherichia coli orE. coli cells having high aspartase activity were immobilized by various methods.
In 1973 we succeeded in the industrial production ofl-aspartic acid usingE. coli cells immobilized with polyacrylamide gel.
For the improvement of this process, we developed a novel technique using κ-carrageenan as the immobilizing matrix forE. coli cells. Further, EAPc-7 strain, having higher aspartase activity, was contracted from the parentE. coli by continuous cultivation with a definite medium. The aspartase activity was about seven times higher than that of the parent cells. In 1982 we changed from the conventional method to the improved method, using EAPc-7 strain immobilized with κ-carrageenan.
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References
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Chibata, I., Tosa, T. & Sato, T. Continuous production of L-aspartic acid. Appl Biochem Biotechnol 13, 231–240 (1986). https://doi.org/10.1007/BF02798461
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DOI: https://doi.org/10.1007/BF02798461
Index Entries
- Production, of L-aspartic acid
- L-aspartic acid, production of
- immobilized aspartase, in production of L-aspartic acid
- aspartase, immobilization of
- aspartase, with polyacrylamide, production of
- immobilized cells, in production of L-aspartic acid
- Escherichia coli ATCC 11303, immobilization ofE. coli ATCC 11303