Effect of glutaraldehyde on the activity of some DNA restriction endonucleases
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The effect of the bifunctional crosslinking reagent glutaraldehyde on the activity of the restriction enzymes Bam HI,Hind III, EcoRI, and Tthlll I was investigated. The four enzymes exhibited differential sensitivity to inactivation. Tthlll I was the most sensitive, with activity losses occurring at levels of 0.0025% and above.Hind III was the most stable of the four and remained fully active at concentrations as high as 0.075%. Addition of BSA to incubation mixtures generally had a stabilizing effect. Implications of these results for the design of glutaraldehyde-based methods for the immobilization of restriction endonucleases are discussed.
Index entriesGlutaraldehyde, effect of on DNA restriction endonucleases restriction endonucleases glutaraldehyde-based methods, for immobilization of restriction endonucleases restriction enzymes
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