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Applied Biochemistry and Biotechnology

, Volume 13, Issue 1, pp 29–35 | Cite as

Effect of glutaraldehyde on the activity of some DNA restriction endonucleases

  • John Olszewski
  • Bruce P. Wasserman
Original Articles

Abstract

The effect of the bifunctional crosslinking reagent glutaraldehyde on the activity of the restriction enzymes Bam HI,Hind III, EcoRI, and Tthlll I was investigated. The four enzymes exhibited differential sensitivity to inactivation. Tthlll I was the most sensitive, with activity losses occurring at levels of 0.0025% and above.Hind III was the most stable of the four and remained fully active at concentrations as high as 0.075%. Addition of BSA to incubation mixtures generally had a stabilizing effect. Implications of these results for the design of glutaraldehyde-based methods for the immobilization of restriction endonucleases are discussed.

Index entries

Glutaraldehyde, effect of on DNA restriction endonucleases restriction endonucleases glutaraldehyde-based methods, for immobilization of restriction endonucleases restriction enzymes 

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Copyright information

© Humana Press Inc. 1986

Authors and Affiliations

  • John Olszewski
    • 1
  • Bruce P. Wasserman
    • 1
  1. 1.Department of Food Science, New Jersey Agricultural Experiment Station, Cook CollegeRutgers UniversityNew Brunswick

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