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Applied Biochemistry and Biotechnology

, Volume 13, Issue 1, pp 1–13 | Cite as

Simple enzyme reactors suitable for the byproduct-free preparation of the aglycones of naturally occurring glycosides under mild conditions

  • P. Turecek
  • F. Pittner
Original Articles

Abstract

Naringinase from Penicillium species—containing α-l-rhamnosidase and β-d-glucosidase activity—immobilized on silicate carriers can be used as a mild and effective means to cleave naturally occurring glycosides into their aglycones and sugar components. The procedure was tested with flavonoid-, anthraquinone-, and steroidglycosides. Since the solubility of such compounds is limited in aqueous solutions, a simple batch procedure has been developed to convert solid substrates suspended in only small amounts of buffer to the desired products with high yields.

Index entries

Immobilized naringinase glycosides aglycones, preparation of glycosides, cleavage of 

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Copyright information

© Humana Press Inc. 1986

Authors and Affiliations

  • P. Turecek
    • 1
  • F. Pittner
    • 2
  1. 1.Institut f. Allgemeine Biochemie der Univ.Wien
  2. 2.Ludwig Boltzmann ForschungsstelleWienAustria

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