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Simple enzyme reactors suitable for the byproduct-free preparation of the aglycones of naturally occurring glycosides under mild conditions

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Abstract

Naringinase from Penicillium species—containing α-l-rhamnosidase and β-d-glucosidase activity—immobilized on silicate carriers can be used as a mild and effective means to cleave naturally occurring glycosides into their aglycones and sugar components. The procedure was tested with flavonoid-, anthraquinone-, and steroidglycosides. Since the solubility of such compounds is limited in aqueous solutions, a simple batch procedure has been developed to convert solid substrates suspended in only small amounts of buffer to the desired products with high yields.

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References

  1. Roitner, M., Schalkhammer, Th., and Pittner, F. (1984),Applied Biochem. and Biotechn.9, 483.

    CAS  Google Scholar 

  2. Weetall, H. (1976), inMethods in Enzymology 44, Mosbach, K., ed., p. 139, Academic, New York, NY.

    Google Scholar 

  3. Pittner, F., Miron, T., Pittner, G., and Wilchek, M. (1980),J. Solid Phase Biochem.5, 167.

    Google Scholar 

  4. Jacobs, S. (1959),Nature 183, 262.

    Article  CAS  Google Scholar 

  5. Habelt, K., and Pittner, F. (1983),Analyt. Biochem.134, 393.

    Article  CAS  Google Scholar 

  6. Wichtl, M. (1971),Die Pharmakognost, Chem. Analyse, Hecht, F., Kaiser, R., and Simon, W., eds., p. 398, Akadem. Verlagsges., Frankfurt.

    Google Scholar 

  7. Wichtl, M., Peithner, G., and Fuchs, L. (1962),Planta Med. 10, 304.

    Article  CAS  Google Scholar 

  8. Christ, B., and Müller, K. H. (1960),Arch. Pharmaz. Ber. Dtsch. Pharmaz. Ges. 293, 1033.

    CAS  Google Scholar 

  9. Wichtl, M. (1971),Die Pharmakognost. Chem. Analyse, Hecht, F., Kaiser, R., and Simon, W., eds., p. 323, Akad. Verlagsges., Frankfurt.

    Google Scholar 

  10. Westphal, O., and Feier, O. (1956),Chem. Ber.89, 582.

    Article  CAS  Google Scholar 

  11. Pittner, F., Miron, T., Pittner, G., and Wilchek, M. (1980),J. Am. Chem. Soc. 102, 2451.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institut f. Allgemeine Biochemie der Univ., Wien

    P. Turecek

  2. Ludwig Boltzmann Forschungsstelle, Währingerstr. 38, A - 1090, Wien, Austria

    F. Pittner

Authors
  1. P. Turecek
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  2. F. Pittner
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Turecek, P., Pittner, F. Simple enzyme reactors suitable for the byproduct-free preparation of the aglycones of naturally occurring glycosides under mild conditions. Appl Biochem Biotechnol 13, 1–13 (1986). https://doi.org/10.1007/BF02798434

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  • DOI: https://doi.org/10.1007/BF02798434

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