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Activity of carboxypeptidase a bound to a modified cellulosic matrix

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Abstract

Carboxypeptidase A immobilized on acid chloride of oxidized cellulose showed the following features: (a) as indicated by the linearity of reaction kinetics, the immobilized enzyme action is not diffusion controlled; (b) greater flow rates are achievable with less clogging during continual usage since the enzyme is attached to a porous screen; (c) ease of handling; and (d) no apparent electrostatic interaction with the support material that is uncharged. The immobilized enzyme retained 60% of the original activity. The half-life of free enzyme was only 20 min, whereas for immobilized enzyme it was enhanced up to 2 h 48 min. It could be recovered and repeatedly used.

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References

  1. Silman, I. H., and Katchalski, E. (1966),Annu. Rev. Biochem. 35, 873.

    Article  CAS  Google Scholar 

  2. Kay, G. (1968),Process Biochem. 3, 36.

    CAS  Google Scholar 

  3. Barker, S. A., and Epton, L. (1970),Process Biochem. 5, 14.

    CAS  Google Scholar 

  4. Volfin, P. (1971),Recherche 2, 571.

    Google Scholar 

  5. Sundaram, P. V., and Laidler, K. J. (1971),Chemistry of the Cell Interface, Part A, Academic, NY, Ch. V.

    Google Scholar 

  6. Goldstein, L., and Katchalski-Katzir, E. (1976), inImmobilized Enzyme Principles, vol. I, Wingard, L. B., Katchalski-Katzir, E., and Goldstein, L., eds., Academic, NY, pp. 1–22.

    Google Scholar 

  7. Goldstein, L. (1970),Methods Enzymol. 19, 935.

    Article  CAS  Google Scholar 

  8. Goldman, R., Goldstein, L., and Katchalski, E. (1971),Biochemical Aspects of Reactions on Solid Supports, Stark, G. R., ed., Academic, NY, p. 1.

    Google Scholar 

  9. Hornby, W. E., Lilly, M. D., and Crook, E. M. (1966),Biochem. J.,98, 420.

    CAS  Google Scholar 

  10. Alexander, B., Rimon, A., and Katchalski, E. (1965),Fed. Proc. 24, 804.

    CAS  Google Scholar 

  11. Bar-Eli, A., and Katchalski, E. (1960),Nature 188, 856.

    Article  CAS  Google Scholar 

  12. Bar-Eli, A., and Katchalski, E. (1963),J. Biol. Chem. 238, 1960.

    Google Scholar 

  13. Weetall, H. H., and Weliky, N. (1966),Anal. Biochem. 14, 159.

    Article  Google Scholar 

  14. Goldman, R., Silman, I. H., Caplan, S., Kedem, O., and Katchalski, E. (1965),Science 150, 758.

    Article  CAS  Google Scholar 

  15. Goldstein, L., Levin, Y., and Katchalski, E. (1964),Biochemistry 3, 1905.

    Article  Google Scholar 

  16. Michael, F., and Evers, J. (1949),Makromol. Chem. 3, 200.

    Article  Google Scholar 

  17. Mitz, M., and Summaria, L. (1961),Nature 189, 576.

    Article  CAS  Google Scholar 

  18. Hornby, W. E., Lilly, M. D., and Crook, E. M. (1968),Biochem. J. 107, 669.

    CAS  Google Scholar 

  19. Dua, R. D., Vasudevan, P., and Kumar, S. (1984),J. Macromol. Sci. Chem. A21(1), 43.

    CAS  Google Scholar 

  20. Singh, M., Ray, A. R., Vasudevan, P., Verma, K., and Guha, S. K. (1978), inDigest of International Symposium and Workshop on Biomed. Eng., in I.I.T., Delhi, India, P. 88.

    Google Scholar 

  21. Nevell, T. P. (1963), inMethods in Carbohydrate Chemistry, vol. 3, Whistler, R. L., ed., Academic, NY, p. 165.

    Google Scholar 

  22. Folk, J. E., and Gladner, J. A. (1958),J. Biol. Chem. 231, 379.

    CAS  Google Scholar 

  23. Dua, R. D., and Srivastava, S. R. (1981),J. Mol. Cat. 10, 253.

    Article  CAS  Google Scholar 

  24. Lineweaver, H., and Burk, D. (1934),J. Am. Chem. Soc. 56, 658.

    Article  CAS  Google Scholar 

  25. Dua, R. D., Vasudevan, P., and Kumar, S.,Biotech. Bioeng., in press.

  26. Kumar, S., Vasudevan, P., and Dua, R. D.,Biotech. Bioeng., Communicated.

  27. Axin, R., Myrin, P. A., and Janson, J. C. (1970),Biopolymers 9, 401.

    Article  Google Scholar 

  28. Dua, R. D., Vasudevan, P. V., and Kumar, S.,J. Macromol. Sci. Chem., Accepted.

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Authors and Affiliations

  1. Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Hauz Khas, 110 016, New Delhi, India

    Sanjay Kumar & R. D. Dua

  2. centre for Rural Development and Appropriate Technology, Indian Institute of Technology, 110 016, New Delhi, India

    Padma Vasudevan

Authors
  1. Sanjay Kumar
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  2. Padma Vasudevan
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  3. R. D. Dua
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Kumar, S., Vasudevan, P. & Dua, R.D. Activity of carboxypeptidase a bound to a modified cellulosic matrix. Appl Biochem Biotechnol 12, 107–119 (1987). https://doi.org/10.1007/BF02798418

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  • DOI: https://doi.org/10.1007/BF02798418

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