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Protein engineering and site-directed mutagenesis

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Abstract

Protein engineering and site-directed mutagenesis is becoming immensely important in both fundamental studies and commercial applications involving proteins and enzymes in biocatalysis. Protein engineering has become a powerful tool to help biochemists and molecular enzymologists elucidate structure-function relationships in enzymic active sites, to understand the intricacies of protein folding and denaturation, and to alter the selectivity of enzymatic catalysis. Commercial applications of engineered enzymes are being developed to increase protein stability, widen or narrow substrate specificity, and to develop novel approaches for use of enzymes in organic synthesis, drug design, and clinical applications. In addition to protein engineering, novel expression systems have been designed to prepare large quantities of genetically engineered proteins. Recent US patents and scientific literature on protein engineering, site-directed mutagenesis, and protein expression systems related to protein engineering are surveyed. Patent abstracts are summarized individually and a list of literature references are given.

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  1. Department of Chemical and Biochemical Engineering, University of Iowa, 52242, Iowa City, IA

    Jonathan S. Dordick

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Dordick, J.S., Backman, K.C., Balakrishnan, R. et al. Patents and literature. Appl Biochem Biotechnol 26, 107–113 (1990). https://doi.org/10.1007/BF02798396

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