Applied Biochemistry and Biotechnology

, Volume 26, Issue 1, pp 73–84 | Cite as

Interactive metabolic regulations during biomethanation ofLeucaena leucocephala

  • Shanthi Krishnan
  • K. Lalitha


Biomethanation of leaves of the legumeL. leucocephala operated in batch reactors at different input volatile solids (12–18 g/L) proceeded in distinct metabolic phases. An initial cellulolytic phase of 4 d was followed by an early and active methanogenic phases (5–21 d) and a terminal phase of low-rate methanogenesis. Hydrolysis of cellulose was concentration-dependent and resulted in increased volatile acid levels. The trend of changes showed some variations at different input volatile solids. The changes in the levels of volatile acids followed an oscillatory pattern. The controlled rate of cellulose hydrolysis, levels of volatile acids, and steady-state levels of soluble carbohydrates and reducing sugars observed during active methanogenesis are indicative of interactive metabolic regulations.

Index entries

Biomethanation and, -metabolic regulations -kinetics -cellulose degradation -steady state parameters and-of L.leucocephala anaerobic digestion ofL. leucocephala anaerobic digestion and, -kinetics in batch reactors and -biomethanation efficiency 


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Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • Shanthi Krishnan
    • 1
  • K. Lalitha
    • 1
  1. 1.Department of Chemistry (Biochemistry)Indian Institute of TechnologyMadrasIndia

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