Skip to main content
SpringerLink
Log in

Stabilization of the structure and unctions of a photosystem i submembrane fraction by immobilization in an albumin-glutaraldehyde matrix

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The effect of immobilization in an albumin-glutaraldehyde crosslinked matrix on the structure and activity of a photosystem I submembrane fraction has been studied. The photosynthetic activity recovered after immobilization was between 35 and 45% of the oxygen-uptake rates of the native material. Resulting oxygen uptake activities found in immobilized photosystem I preparations with methylviologen as acceptor were as high as 270 μmol O2 (mg Chl h)-1, An enhancement of photosystem I electron transfer, which is produced by incubation of thylakoid membranes at temperatures above 30 °C, was detected in native submembrane fractions, but not in the immobilized preparations. It is suggested that the increased activity at high temperature results from conformational modifications not allowed in the immobilization matrix. The insensitivity of immobilized photosystem I particles to prolonged storage at 4°C and to strong light exposure, as well as their high electron-transfer rates, demonstrates that the immobilization procedure used can be successfully applied to submembrane fractions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kayano, H., Matsunaga, T., Karube, I., and Suzuki, S. (1981),Biotechnol. Bioeng. 23, 2283–2291.

    Article  CAS  Google Scholar 

  2. De la Rosa, M. A., Rao, K. K., and Hall, D. O. (1986),Photobiochem. Photobiophys. 11, 173–187.

    Google Scholar 

  3. Sangeeta, A. and Nair, P. M. (1986),Photobiochem. Photobiophys. 13, 73–84.

    CAS  Google Scholar 

  4. Lemieux, S. and Carpentier, R.(1988),Photochem. Photobiol. 48, 115–121.

    Article  CAS  Google Scholar 

  5. Cocquempot, M. F., Hervagault, J. F., and Thomas, D. (1986),Enzyme Microb. Technol. 9, 533–536.

    Article  Google Scholar 

  6. Pan, R. L., Fan, I.-J., Bhardwaj, R., and Gross, E. L. (1982),Photochem. Photobiol. 35, 155–164.

    Google Scholar 

  7. Pan, R. L., Bhardwaj, R., and Gross, E. L. (1983),J. Chem. Tech. Biotechnol. 33A, 39–48.

    CAS  Google Scholar 

  8. Powles, S.B. (1984),Ann. Rev. Plant Physiol. 35, 15–44.

    CAS  Google Scholar 

  9. Cornic, G. and Miginiac-Maslow, M. (1985),Plant Physiol. 78, 724–729.

    CAS  Google Scholar 

  10. Katoh, S. and San Pietro, A. (1967),Arch. Biochem. Biophys. 122, 144–152.

    Article  CAS  Google Scholar 

  11. Pearcy, R. W., Berry, J. A., and Fork, D. C. (1977),Plant Physiol. 59, 873–878.

    Article  CAS  Google Scholar 

  12. Armond, P. A., Schreiber, U., and Bjorkman, O. (1978),Plant. Physiol. 61, 411–415.

    CAS  Google Scholar 

  13. Stidham, M. A., Uribe, E. G., and Williams, G. J. (1982),Plant Physiol. 69, 929–934.

    Article  CAS  Google Scholar 

  14. Thomas, P. G., Quinn, P. S., and Williams, W. P. (1986),Planta 167, 133–139.

    Article  CAS  Google Scholar 

  15. Cocquempot, M.-F., Thomasset, B., Barbotin, J. N., Gellf, G., and Thomas, D. (1981),Eur. J. Appl. Microbiol. Biotechnol. 11, 193–198.

    Article  CAS  Google Scholar 

  16. Thomasset, B., Barbotin, J.-N., Thomas, D., Thomasset, T., Vejux, A., and Jeanfils, J. (1983),Biotechnol. Bioeng. 25, 2453–2468.

    Article  CAS  Google Scholar 

  17. Peters, F. A. L. J., Van Wielink, J. E., Wong Fong Sang, H. W., De Vries, S., and Kraayenhof, R. (1983),Biochim. Biophys. Acta 722, 460–470.

    Article  CAS  Google Scholar 

  18. Arnon, D. I. (1949),Plant Physiol 24, 1–15.

    CAS  Google Scholar 

  19. Thomasset, B., Thomasset, T., Vejux, A., Jeanfils, J., Barbotin, J.-N., and Thomas, D. (1982),Plant Physiol. 70, 714–722.

    CAS  Google Scholar 

  20. Carpentier, R., Larue, B., and Leblanc, R. M. (1984),Arch. Biochem. Biophys. 338, 534–543.

    Article  Google Scholar 

  21. Jeanfils, J., Cocquempot, M.-F., and Thomas, D. (1986),Enzyme Microb. Technol. 8, 157–160.

    Article  CAS  Google Scholar 

  22. Carpentier, R., Leblanc, R. M., and Mimeault, M. (1988),Biotechnol. Bioeng. 32, 64–67.

    Article  CAS  Google Scholar 

  23. Isaakidou, J. and Papageorgiou, G. C. (1979),Arch. Biochem. Biophys. 195, 280–287.

    Article  CAS  Google Scholar 

  24. Hardt, H. and Kok, B. (1976),Biochim. Biophys. Acta 449, 125–135.

    Article  CAS  Google Scholar 

  25. Inoue, K., Sakurai, H., and Hiyama, T. (1986),Plant Cell Physiol. 27, 961–968.

    CAS  Google Scholar 

  26. Carpentier, R. and Lemieux, S. (1987),App. Biochem. Biotechnol. 15, 107–117.

    CAS  Google Scholar 

  27. Carpentier, R., Leblanc, R. M., and Mimeault, M. (1987),Enzyme Microb. Technol. 9, 489–493.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center de recherche en photobiophysique, Université du Québec a, Trois-Rivières, C.P. 500, G9A 5H7, Trois-Rivières, Québec, Canada

    Danielle Bonenfant & Robert Carpenter

Authors
  1. Danielle Bonenfant
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert Carpenter
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bonenfant, D., Carpenter, R. Stabilization of the structure and unctions of a photosystem i submembrane fraction by immobilization in an albumin-glutaraldehyde matrix. Appl Biochem Biotechnol 26, 59–71 (1990). https://doi.org/10.1007/BF02798393

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02798393

Index entries

Search

Navigation