Applied Biochemistry and Biotechnology

, Volume 26, Issue 1, pp 35–44 | Cite as

Synthesis of monobutyrylglycerol by transesterification with soluble and immobilized lipases

  • Cristina Otero
  • Eitel Pastor
  • Antonio Ballesteros


Transesterification between ethyl butyrate and glycerol using very different lipase preparations (native, modified, and immobilized) in a two-phase system—no extra solvent added—has been investigated. Optimal conversion was obtained with the presence of 5% water in the reaction mixture. Only monobutyrin was produced in all conditions tested. The best enzyme preparations were nativeCandida cylindracea lipase,Mucor miehei lipase immobilized on a phenol-formaldehyde exchange resin (Lipozyme™), andC. cylindracea lipase immobilized on Celite.

Index entries

Monobutyrin ethyl butyrate, transesterification of glycerol esterification Candida cylindracea and pancreatic lipases lipases immobilized on Celite and on agarose polyethylene glycol modified-lipase 


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Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • Cristina Otero
    • 1
  • Eitel Pastor
    • 1
  • Antonio Ballesteros
    • 1
  1. 1.Instituto de CatalisisCSICMadridSpain

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