Applied Biochemistry and Biotechnology

, Volume 9, Issue 1, pp 81–93 | Cite as

Transport of acetylcholine in a membrane

Laminate model of the neuromuscular junction
  • Sachio Hirose
  • Wolf R. Vieth
Original Articles


A laminate model of the cleft-plus-postsynaptic membrane structure of the neuromuscular junction was studied. In order to prepare a model of the postsynaptic membrane, the properties of acetylcholine (Ach) receptor-rich vesicles purified from Torpedo fish were measured. Immobilization of vesicles was demonstrated by various methods, in particular, by investigating collagen and carrageenan matrices as models of the fluidfilled fibrous matrix of the cleft. It was found that a laminated system employing a liquid membrane-containing vesicle suspension, together with a swollen collagen membrane, is an appropriate model for examining important transport/reception aspects of the cleft-plus-postsynaptic membrane structure.

Combined transport with immobilization of Ach in the liquid membrane system was elucidated and effective diffusivities in the vesicle suspension layer were calculated. Effective diffusivities of the composite system simulating the cleft and the postsynaptic membrane were evaluated as well. These data illustrate the importance of penetrant immobilization in retarding the diffusion process during neurotransmission.

Index Entries

Transport, of acetylcholine in a membrane acetylcholine, trans-port in a membrane membrane, acetylcholine transport in 


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  1. 1.
    Noback, C. R., and Demarest, R. J., eds. (1981),The Human Nervous System, McGraw-Hill, New York.Google Scholar
  2. 2.
    O’Brien, R. D. (ed.) (1980),The Receptors, Plenum, New York.Google Scholar
  3. 3.
    Lester, H. A. (1977),Sci. Amer. 236, 106.CrossRefGoogle Scholar
  4. 4.
    Lindstrom, J., Anholt, R., Einarson, B., Engel, A., Osame, M., and Montal, M. (1980),J. Biol. Chem. 225, 8340.Google Scholar
  5. 5.
    Anholt, R., Lindstrom, J., and Montai, M. (1980),Eur. J. Biochem. 109, 481.CrossRefGoogle Scholar
  6. 6.
    Elliott, J., Blanchard, S. G., Wu, W., Miller, J., Strader, C. D., Hartig, P., Moore, H. P., Racs, J., and Raftery, M. A. (1980),Biochem. J. 185, 667.Google Scholar
  7. 7.
    Hirose, S., Yasukawa, E., Hayashi, M., and Vieth, W. R. (1982),J. Membrane Sci. 11(2), 177.CrossRefGoogle Scholar
  8. 8.
    Barrie, J. A., Levine, J. D., Michaels, A. S., and Wong, P. (1963),Trans. Faraday Soc. 59, 869.CrossRefGoogle Scholar
  9. 9.
    Hirose, S., Vieth, W. R., and Takao, M. (1983),J. Molec. Catal. 18, 11.CrossRefGoogle Scholar
  10. 10.
    Eccles, J. C., and Jaeger, J. C. (1958),Proc. R. Soc. B148, 38.Google Scholar

Copyright information

© Humana Press Inc 1984

Authors and Affiliations

  • Sachio Hirose
    • 1
  • Wolf R. Vieth
    • 2
  1. 1.Central Research LaboratoriesMitsubishi Petrochemical Co., Ltd.IbarakiJapan
  2. 2.Department of Chemical and Biochemical EngineeringRutgers UniversityPiscataway

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