Abstract
A heteroenzyme conjugate retaining activities of two component enzymes from trypsin and chymotrypsin was prepared using N-succinimidyl pyridyl dithiopropionate as crosslinking reagent. The conjugate bound to both trypsin and chymotrypsin affinity columns. Trypsin and chymotrypsin were linked in the ratio of 1:1 on mol basis. The conjugate, when treated with dimethyladipimidate, showed decreased autolysis of its trypsin component.
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Rajput, Y.S., Gupta, M.N. A conjugate of trypsin and chymotrypsin. Appl Biochem Biotechnol 16, 201–210 (1987). https://doi.org/10.1007/BF02798367
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DOI: https://doi.org/10.1007/BF02798367