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A conjugate of trypsin and chymotrypsin

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Abstract

A heteroenzyme conjugate retaining activities of two component enzymes from trypsin and chymotrypsin was prepared using N-succinimidyl pyridyl dithiopropionate as crosslinking reagent. The conjugate bound to both trypsin and chymotrypsin affinity columns. Trypsin and chymotrypsin were linked in the ratio of 1:1 on mol basis. The conjugate, when treated with dimethyladipimidate, showed decreased autolysis of its trypsin component.

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References

  1. Shier, W. T. (1985),Meth. Enzymol. 112, 248–258.

    Article  CAS  Google Scholar 

  2. Voller, A., Bidwell, D. M., and Bartlett, A. (1976),Bull. World Health Organ. 53, 55–65.

    CAS  Google Scholar 

  3. Yoshitake, S., Yamada, Y., Ishikawa, E., and Masseyeff, R. (1979),Eur. J. Biochem. 101, 395–399.

    Article  CAS  Google Scholar 

  4. Ikura, K., Okumura, K., Sasaki, R., and Chiba, H. (1984),Agric. Biol. Chem. 48, 355–364.

    CAS  Google Scholar 

  5. Wang, D. (1979),Biochem. 18, 4449–4452.

    Article  CAS  Google Scholar 

  6. Rajput, Y. S. and Ganguli, N. C. (1982),Asian J. Dairy Res. 1, 13–16.

    CAS  Google Scholar 

  7. Carlsson, J., Drevin, H., and Axen, R. (1978),Biochem. J. 173, 723–737.

    CAS  Google Scholar 

  8. Decker, L. A. (ed.) (1977a),Worthington enzyme manual, 221–224, Worthington Biochemical Corp., Freehold, NJ, USA.

    Google Scholar 

  9. Decker, L. A. (ed.) (1977b),Worthington enzyme manual, 215–220, Worthington Biochemical Corp., Freehold, NJ, USA.

    Google Scholar 

  10. Stuchbury, T., Shipton, M., Norris, R., Malthouse, J. P. G., Brocklehurst, K., Herbert, J. A. L., and Suschitzky, H. (1975),Biochem. J. 151, 417–432.

    CAS  Google Scholar 

  11. Bradford, M. M. (1976),Anal. Biochem. 72, 248–254.

    Article  CAS  Google Scholar 

  12. Erlanger, B. F., Kokowsky, N., and Cohen, W. (1961),Arch. Biochem. Biophys. 95, 271–278.

    Article  CAS  Google Scholar 

  13. Rick, W. (1974), inMethods of Enzymatic Analysis (Bergmeyer, U. ed.) vol. 2, 2nd ed., pp. 1013–1024, Verlag Chemie Gmb-H., Weinheim.

    Google Scholar 

  14. Okumura, K., Ikura, K., Yoshikawa, M., Sasaki, R., and Chiba, H. (1978),Agric. Biol. Chem. 48, 2435–2440.

    Google Scholar 

  15. Kincaid, R. L. (1984),Biochem. 23, 1143–1147.

    Article  CAS  Google Scholar 

  16. Rajput, Y. S. and Gupta, M. N. (1987),Enz. Microb. Technol. 9, 161–163.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Animal Biochemistry Division, National Dairy Research Institute, 132 001, Karnal

    Y. S. Rajput

  2. Chemistry Department, Indian Institute of Technology, 110 016, New Delhi, India

    M. N. Gupta

Authors
  1. Y. S. Rajput
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  2. M. N. Gupta
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Rajput, Y.S., Gupta, M.N. A conjugate of trypsin and chymotrypsin. Appl Biochem Biotechnol 16, 201–210 (1987). https://doi.org/10.1007/BF02798367

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  • DOI: https://doi.org/10.1007/BF02798367

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