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Use of enzymes in peptide synthesis

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Abstract

Recent experiments in several laboratories have emphasized the benefits of proteolytic enzymes as effective catalysts for the formation of peptide bonds for synthesis and semisynthesis. This review summarizes successful applications in both stepwise synthesis for small peptides and fragment condensation to produce large polypeptides and proteins.

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Author notes

  1. Motonori Ohno

    Present address: Laboratory of Enzyme Chemistry, Faculty of Science, Kyushu University, Fukuoka, Japan

  2. Fred Widmer

    Present address: Carlsberg Biotechnology, LTD, Tagensvej 16, DK-2200, Copenhagen N, Denmark

Authors and Affiliations

  1. Laboratory of Chemical Biology, National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases, USA

    Irwin M. Chaiken, Akira Komoriya, Motonori Ohno & Fred Widmer

  2. Laboratory of Chemoprevention, National Cancer Institute, National Institutes of Health, 20205, Bethesda, MD, USA

    Akira Komoriya

Authors
  1. Irwin M. Chaiken
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  2. Akira Komoriya
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  3. Motonori Ohno
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  4. Fred Widmer
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Chaiken, I.M., Komoriya, A., Ohno, M. et al. Use of enzymes in peptide synthesis. Appl Biochem Biotechnol 7, 385–399 (1982). https://doi.org/10.1007/BF02798320

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