Applied Biochemistry and Biotechnology

, Volume 7, Issue 4, pp 307–313 | Cite as

Rapid determination of anti-enzyme titer in serum using wholeStaphylococcus aureus cells

  • Ronald W. McClard
Original Articles


Heat-killed, formalin-fixedStaphyloccous aureus (Cowan) cells are shown to be an excellent immunoadsorbent for quickly and accurately measuring the titer of antibodies directed against enzymes. The simple procedure is illustrated by following the course of immunization of a rabbit against mouse uridine 5’t -monophosphate synthase. The antibody titer of a serum sample can be determined in roughly 1 h plus the time to perform the assays of enzyme (antigen) activity.

Index Entries

Staphyloccus aureus, use of in determination of antibody titer anti-enzyme titer, determination of using wholeStaphyloccus aureus cells immunoadsorbent, solid-phase uridine 5’t-monophospate synthase rapid determination of anti-enzyme titer serum, rapid assay of anti-enzyme titer in 


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Copyright information

© Humana Press Inc 1982

Authors and Affiliations

  • Ronald W. McClard
    • 1
  1. 1.Department of BiochemistryUniversity of North Carolina, School of MedicineChapel Hill

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