Abstract
This paper describes an affinity chromatography procedure to purify an urate binding protein from human serum. The specific ligand was 8-amino-2,6-dihydroxypurine bound to Sepharose through the amino group. The specific elution was obtained with an uric acid or allopurinol solution. Electrophoretic analysis of the eluted protein shows a single sharp band with an α2-globulin mobility. Molecular weight, determined by gel filtration, is approximately 70,000 daltons.
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Mazzoni, M.R., Martini, C., Segnini, D. et al. Isolation of an urate-binding protein by affinity chromatography. Appl Biochem Biotechnol 7, 189–194 (1982). https://doi.org/10.1007/BF02798296
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DOI: https://doi.org/10.1007/BF02798296