Abstract
Whole cells ofTrigonopsis variabilis were immobilized by entrapment in Ca2+-alginate and used for the production of α-keto acids from the corresponding D-amino acids. The D-amino acid oxidase within the immobilized cells has a broad substrate specificity. Hydrogen peroxide formed in the enzymatic reaction was efficiently hydrolyzed by manganese oxide co-immobilized with the cells. The amino acid oxidase activity was assayed with a new method based on reversed-phase HPLC. Oxygen requirements, bead size, concentration of cells in the beads, flow rate, and other factors were investigated in a “ trickle-bed ” reactor.
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Brodelius, P., Nilsson, K. & Mosbach, K. Production of α-keto acids Part I. Immobilized cells ofTrigonopsis variabilis containing D-amino acid oxidase. Appl Biochem Biotechnol 6, 293–307 (1981). https://doi.org/10.1007/BF02798280
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DOI: https://doi.org/10.1007/BF02798280
Index Entries
- Production of α-keto acids
- α-keto acids, production of
- immobilized cells, in the production of α-keto acids
- Trigonopsis variabilis, in the production of α-keto acids
- D-amino acid oxidase, in α-keto acid production
- oxidase, D-aminoacid, in α-keto acid production
- manganese oxide, co-immobilized with whole cells
- liquid chromatography, assay of D-amino acid oxidase activity
- chromatography, liquid, of D-amino acid oxidase activity