In Vitro

, Volume 12, Issue 8, pp 595–598 | Cite as

The co-carcinogenic activity of 4-nitropyridine-1-oxide (4-npo) and prevention of transformation by type-specific anti-viral antibodies

  • Paul J. Price
  • Angela E. Auletta
  • Martin P. King
  • Patricia M. Hugunin
  • Robert J. Huebner


Fischer rat embryo cells chronically infected with Rauscher murine leukemia virus, and known to be sensitive to transformation by potent chemical carcinogens, were transformed by the weak carcinogen 4-nitropyridine-1-oxide. Transformed cells grew in semi-solid agar and produced tumors in newborn Fischer rats. Transformation was inhibited by antisera specific for the ecotropic Rauscher murine leukemia virus, but not by antisera of equal toxicity specific for xenotropic Swiss mouse AT-124 virus.

Key words

inhibition of transformation co-carcinogenesis transformation assay Fischer rat embryo cells 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Nakahara, W., F. Fukuoka, and T. Sugimura. 1957. Carcinogenic action 4-nitroquinoline-1-oxide. Gann 48:129–137.Google Scholar
  2. 2.
    Hartman, P. E., K. Levine, Z. Hartman, and H. Berger. 1971. Hycanthone: a frameshift mutagen. Science 172:1058–1060.PubMedCrossRefGoogle Scholar
  3. 3.
    Stitch, H. F., R. H. C. San, and Y. Kawazoe. 1971. DNA repair synthesis in mammalian cells exposed to a series of oncogenic and non-oncogenic derivatives of 4-nitroquinoline-1-oxide. Nature 229:416–419.CrossRefGoogle Scholar
  4. 4.
    Epstein, S. S., and I. B. Saporoschetz. 1968. On the association between lysogeny and carcinogenicity in nitroquinolines and related compounds. Experentia 24:1245–1248.CrossRefGoogle Scholar
  5. 5.
    Minako, N., and T. Sugimura. 1972. Sensitivity of repair-deficient mutants and similar mutants to 4-nitroquinoline-1-oxide, 4-nitropyridine-1-oxide, and their derivatives. Cancer Res. 32:2369–2374.Google Scholar
  6. 6.
    Sato, H., and T. Kuroki. 1966. Malignizationin vitro of hamster embryonic cells by chemical carcinogens. Proc. Jap. Acad. 42:1211–1216.Google Scholar
  7. 7.
    Freeman, A. E., H. J. Igel, and P. J. Price. 1975. Carcinogenesisin vitro. I.In vitro transformation of rat embryo cells: correlation with the known tumorigenic activities of chemicals in rodents. In Vitro 11:107–116.PubMedCrossRefGoogle Scholar
  8. 8.
    Kelloff, G. J., M. Hatanaka, and R. V. Gilden. 1972. Assay of C-type virus infectivity by measurement of RNA-dependent DNA polymerase activity. Virology 48:266–269.PubMedCrossRefGoogle Scholar
  9. 9.
    Macpherson, I. 1973. Soft agar techniques. In: P. F. Kruse, Jr., and M. K. Patterson, Jr. (Eds.),Tissue Culture Methods and Applications, Academic Press, New York, pp. 276–280.Google Scholar
  10. 10.
    Sanford, K. K., W. R. Earle, V. J. Evans, H. K. Waltz, and J. E. Shannon. 1951. The measurement of proliferation in tissue cultures by enumeration of cell nuclei. J. Natl. Cancer Inst. 11:773–795.PubMedGoogle Scholar
  11. 11.
    Price, P. J., W. A. Suk, R. L. Peters, and R. J. Huebner. 1976. Enhancement of chemical transformation of rat cells by infection with xenotropic type “C” RNA virus. Submitted for publication.Google Scholar
  12. 12.
    Freeman, A. E., R. V. Gilden, M. L. Vernon, R. G. Wolford, P. M. Hugunin, and R. J. Huebner. 1973. 5-Bromo-2′-deoxyuridine potentiation of transformation of rat cells inducedin vitro by 3-methylcholanthrene: induction of rat leukemia virus gs antigen in transformed cells. Proc. Natl. Acad. Sci. U.S.A. 70:2415–2419.PubMedCrossRefGoogle Scholar
  13. 13.
    Price, P. J., T.M. Bellew, M. P. King, A. E. Freeman, R. V. Gilden, and R. J. Huebner. 1976. Prevention of viral-chemical co-carcinogenesisin vitro by type-specific anti-viral antibody. Proc. Natl. Acad. Sci. U.S.A. 73:152–155.PubMedCrossRefGoogle Scholar

Copyright information

© Tissue Culture Association 1976

Authors and Affiliations

  • Paul J. Price
    • 1
  • Angela E. Auletta
    • 1
  • Martin P. King
    • 1
  • Patricia M. Hugunin
    • 1
  • Robert J. Huebner
    • 1
    • 2
  1. 1.Microbiological Associates-Torrey Pines Research CenterLa Jolla
  2. 2.National Cancer InstituteBethesda

Personalised recommendations