Cell Biophysics

, Volume 14, Issue 1, pp 17–26 | Cite as

TMA-DPH A fluorescent probe of membrane dynamics in living cells

How to use it in phagocytosis
  • Dominique Illinger
  • Martial Kubina
  • Guy Duportail
  • Philippe Poindron
  • Jacques Bartholeyns
  • Jean-Georges Kuhry
Original Articles Cell


Trimethylammonium-diphenylhexatriene (TMA-DPH), a hydrophobic fluorescent probe, has been shown in earlier studies to possess a variety of particular properties in interaction with intact living cells —specific and rapid incorporation into the plasma membrane and partition equilibrium between the membranes and the buffer. These properties offer promising applications in membrane fluidity studies and in monitoring exocytosis kinetics. Furthermore, these properties offer a method described here for quantitative monitoring of phago-cytosis kinetics, by means of simple fluorescence intensity measurements. This method is original in that it evaluates only the particles which have actually been internalized by phagocytosis, and not those adsorbed on the cell surface, and that it gives quantitative information on the amount of plasma membrane involved in the process. It has been tested on mouse bone marrow macrophages.

Index Entries

Phagocytosis TMA-DPH fluorescent probe for membrane dynamics macrophages 


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Copyright information

© The Humana Press Inc. 1989

Authors and Affiliations

  • Dominique Illinger
    • 1
  • Martial Kubina
    • 2
  • Guy Duportail
    • 2
  • Philippe Poindron
    • 1
  • Jacques Bartholeyns
    • 1
  • Jean-Georges Kuhry
    • 2
  1. 1.Department d’Immunologie UA 491 du CNRSUniversite Louis PasteurStrasbourg CedexFrance
  2. 2.Laboratoire de Biophysique UA 491 du CNRSUniversite Louis PasteurStrasbourg CedexFrance

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