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Biological Trace Element Research

, Volume 16, Issue 3, pp 177–188 | Cite as

Subchronic treatment with vanadate does not potentiate the toxicity of cardiac glycosides

  • E. MacDonald
  • H. Lihtamo
  • Kaisa Hellevuo
  • H. Komulainen
Article

Abstract

Since it has been claimed that vanadate is an endogenous regulator of Na/K-ATPase activity and that it potentiates the toxicity of cardiac glycosides, we were alarmed to discover that certain Finnish physicians were prescribing vanadate in combination with other trace minerals to elderly patients for many different chronic diseases (e.g., cancer, rheumatism). To study the interaction of vanadate and cardiac glycosides, we fed vanadate in the drinking water (25 μg/mL) to guinea pigs for 20 d, and studied either their sensitivity to the acute toxicity of the cardiac glycoside ouabain or whether the vanadate would influence the subacute toxicity of ouabain. Vanadate had no influence on the toxicity of ouabain either acute or subchronically administered, nor was there any sign of inhibition of Na/K-ATPase activity as measured by86Rb-uptake into intact erythrocytes (RBCs), RBC content of sodium or potassium or Na/K-ATPase activity in RBC membranes prepared from the vanadate-treated guinea pigs. Vanadate had been absorbed in substantial quantities from the gastrointestinal tract, since serum, heart, liver, and especially kidney contained measurable amounts of vanadium in contrast to controls, but it is concluded that this vanadate is not in a biologically active form.

Index Entries

Vanadate administration cardiac glycoside toxicity Na/K-ATPase inhibition by vanadate, ouabain vanadium in tissues 

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Copyright information

© The Humana Press Inc. 1988

Authors and Affiliations

  • E. MacDonald
    • 1
  • H. Lihtamo
    • 2
  • Kaisa Hellevuo
    • 1
    • 3
  • H. Komulainen
    • 1
    • 4
  1. 1.Department of Pharmacology and Toxicology HygieneUniversity of KuopioKuopio 21Finland
  2. 2.Environmental HygieneUniversity of KuopioKuopio 21Finland
  3. 3.Research Laboratories of Finnish State Alcohol Co.ALKO Ltd.Helsinki
  4. 4.Department of Environmental Hygiene and ToxicologyNational Public Health LaboratoryKuopioFinland

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