Biological Trace Element Research

, Volume 12, Issue 1, pp 167–183 | Cite as

Contact microscopy with synchrotron radiation

  • Barbara J. Panessa-Warren
Applications of Synchrotron Radiation Review


Soft X-ray contact microscopy with synchrotron radiation offers the biologist, and especially the microscopist, a way to morphologically study specimens that could not be imaged by conventional TEM, STEM, or SEM methods (i.e., hydrated samples, samples easily damaged by an electron beam, electron-dense samples, thick specimens, unstained, low-contrast specimens) at spatial resolutions approaching those of the TEM, with the additional possibility to obtain compositional (elemental) information about the sample as well. Although flash X-ray sources offer faster exposure times, synchrotron radiation provides a highly collimated, intense radiation that can be tuned to select specific discrete ranges of X-ray wavelengths or specific individual wavelengths that optimize imaging or microanalysis of a specific sample. This paper presents an overview of the applications of X-ray contact microscopy to biological research and some current research results using monochromatic synchrotron radiation to image biological samples.

Index Entries

Biological soft X-ray contact microscopy X-ray resists absorption edge imaging synchrotron radiation 


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Copyright information

© The Humana Press Inc 1987

Authors and Affiliations

  • Barbara J. Panessa-Warren
    • 1
    • 2
  1. 1.Department of Allied Health ResourcesState University of New York, Stony BrookStony Brook
  2. 2.Instrumentation DivisionBrookhaven National LaboratoryUpton

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