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In Vitro - Plant

, Volume 18, Issue 8, pp 708–714 | Cite as

Development of tetraploidy in V79 spheroids

  • P. L. Olive
  • J. C. Leonard
  • R. E. Durand
Article

Summary

Chinese hamster V-79-171 cells, when placed in suspension culture, spontaneously form multicell spheroids. As the spheroids enlarge the fraction of polyploid (predominantly tetraploid) cells increases and can approach 100% in very large spheroids. Spheroid size, rather than age, seems to be a major determinant for increased ploidy. When cell separation techniques were used to select enriched populations of diploid and tetraploid cells, the growth rate and plating efficiency of the diploid cells was always marginally higher, and they gradually became predominant in mixed monolayer cultures. Cloned tetraploid cells, however, generally remained quite stable, and no consistent ploidy dependent changes in radiosensitivity were observed relative to normal, diploid cell lines.

Key words

spheroids tetraploidy flow cytometry 

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Copyright information

© Tissue Culture Association, Inc 1982

Authors and Affiliations

  • P. L. Olive
    • 1
  • J. C. Leonard
    • 1
  • R. E. Durand
    • 1
  1. 1.Section of RadiobiologyThe Johns Hopkins Oncology CenterBaltimore

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