In Vitro - Plant

, Volume 18, Issue 8, pp 708–714 | Cite as

Development of tetraploidy in V79 spheroids

  • P. L. Olive
  • J. C. Leonard
  • R. E. Durand


Chinese hamster V-79-171 cells, when placed in suspension culture, spontaneously form multicell spheroids. As the spheroids enlarge the fraction of polyploid (predominantly tetraploid) cells increases and can approach 100% in very large spheroids. Spheroid size, rather than age, seems to be a major determinant for increased ploidy. When cell separation techniques were used to select enriched populations of diploid and tetraploid cells, the growth rate and plating efficiency of the diploid cells was always marginally higher, and they gradually became predominant in mixed monolayer cultures. Cloned tetraploid cells, however, generally remained quite stable, and no consistent ploidy dependent changes in radiosensitivity were observed relative to normal, diploid cell lines.

Key words

spheroids tetraploidy flow cytometry 


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  1. 1.
    Sutherland, R. M.; Durand, R. E. Radiation response of multicell spheroids: An in vitro tumor model. Curr. Top. Radiat. Res. Quart. 11: 87–139; 1976.Google Scholar
  2. 2.
    Sutherland, R. M. The multicellular spheroid system as a tumor model for studies of radiation sensitizers. Pharmacol. Ther. 8: 105–123; 1980.CrossRefGoogle Scholar
  3. 3.
    Durand, R. E.; Olive, P. L. Evaluation of nitroheterocyclic radiosensitizers using spheroids. Lett, J., ed. Advances in Radiation Biology. Vol. 9. New York: Academic Press, 1981: 79–104.Google Scholar
  4. 4.
    Olive, P. L.; Durand, R. E. Uptake and mutagenicity of AF-2 in Chinese hamster V-79 spheroids under aerobic and hypoxic conditions. Environ. Mutagen. 3: 659–670; 1981.CrossRefGoogle Scholar
  5. 5.
    Durand, R. E. Isolation of cell populations from in vitro tumor models according to sedimentation velocity. Cancer Res. 35: 1295–1300; 1975.PubMedGoogle Scholar
  6. 6.
    Durand, R. E. Cell cycle kinetics in anin vitro tumor model. Cell Tissue Kinet. 9: 403–412; 1976.PubMedGoogle Scholar
  7. 7.
    Landry, J.; Freyer, J. P.; Sutherland, R. M. Shedding of mitotic cells from the surface of multicell spheroids during growth. J. Cell Physiol. 106: 23–32; 1981.PubMedCrossRefGoogle Scholar
  8. 8.
    Freyer, J.; Sutherland, R. M. Selective dissociation and characterization of cells from different regions of multicell tumor spheroids. Cancer Res. 40: 3956–3965; 1980.PubMedGoogle Scholar
  9. 9.
    Vindelov, L. L. Flow microfluorometric analysis of nuclear DNA in cells from solid tumors and cell suspensions. A new method for rapid isolation and staining of nuclei. Virchows Arch. [Cell Pathol.] 24: 227–242; 1977.Google Scholar
  10. 10.
    Oksala, T. Mitotic abnormalities and cancer. German, J. ed. Chromosomes and cancer. New York: John Wiley and Sons; 1974: 239–261.Google Scholar
  11. 11.
    Barranco, S. C.; Shilkun, K.; Nichols, S.; Boerwinkle, W. R.; Adams, E. G.; Bhuyan, B. K. Changes in DNA distributions and ploidy of CHO cells as a function of time in culture. In Vitro 17: 730–734; 1981.PubMedCrossRefGoogle Scholar
  12. 12.
    Brodsky, W.; Uryvaeva, I. V. Cell polyploidy: Its relation to tissue growth and function. Inter. Rev. Cytol. 50: 275–332; 1978.CrossRefGoogle Scholar

Copyright information

© Tissue Culture Association, Inc 1982

Authors and Affiliations

  • P. L. Olive
    • 1
  • J. C. Leonard
    • 1
  • R. E. Durand
    • 1
  1. 1.Section of RadiobiologyThe Johns Hopkins Oncology CenterBaltimore

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