Human pancreatic adenocarcinoma: In vitro and in vivo morphology of a new tumor line established from ascites
- 285 Downloads
A human pancreatic tumor cell line has been established from the ascites of a patient with histopathologically confirmed adenocarcinoma of the head of the pancreas and maintained for more than 12 months in the laboratory. Epitheloid tumor cell colonies, which resulted from primary tissue cultures of the ascitic cell component, were mechanically isolated by needle micromanipulation. Tumorigenicity was proven in athymic nude mice. Morphologically the pancreatic tumor epithelial cells grew to confluency with moderately tight adhesion to the culture plastic surface and with free-floating cells in the medium. Upon re-establishment of the tumoral xenograft in tissue culture, the epithelial cells retained their original morphology. Histologically the tumor grown in nude mice exhibited prototypic characteristics of the primary adenocarcinoma in the patient, producing abundant mucin and displaying a broad spectrum of glandular differentiation, which ranged from well to poorly differentiated adenocarcinomas with occasionally localized lymphocytic infiltrations. Furthermore, the tumor expressed carcinoembryonic antigen and human pancreas cancer associated antigen. This tumor line, designated AsPC-1, has been cultured for at least 10 passages in vitro and 3 in vivo. It represents a new model for human pancreatic cancer.
Key wordsascites epithelial cells human pancreatic adenocarcinoma
Unable to display preview. Download preview PDF.
- 10.Fitzgerald, P. J.; Sharkey, F. E.; Fogh, J.; Varricchio, F.; Cubilla, A.; Pour, P. The nude mouse as a tissue amplifier for studies of experimental and human pancreas cancer. Fogh, J.; Giovanella, B. C. eds. The nude mouse in experimental and clinical research. New York: Academic Press; 1978: 267–279.Google Scholar
- 14.Hayflick, L. Screening tissue culture for mycoplasma infections. Kruse, R. J., Jr.; Patterson, M. K., Jr. eds. Tissue culture methods and applications. New York: Academic Press; 1973: 722–728.Google Scholar
- 15.Horoszewicz, J. S.; Leong, S. S.; Chu, T. M.; Wajsman, A.; Friedman, M.; Papsidero, L. D.; Kim, U.; Chai, L. S.; Arya, S. K.; Sandberg, A. A. The LNCaP cell line—A new model for studies on human prostatic carcinoma. Murphy, G. P. ed. Models for prostate cancer. New York: Alan R. Liss, Inc.; 1980: 115–132.Google Scholar
- 20.Sykes, J. A. Separation of tumor cells from fibroblasts. Fogh, J. ed. Human tumor cells in vitro. New York: Plenum Press; 1975: 1–22.Google Scholar
- 21.Sharkey, F. E.; Fogh, J. M.; Hajdu, S. I.; Fitzgerald, P. J.; Fogh, J. Experience in surgical pathology with human tumor grown in nude mouse. Fogh, J.; Giovanella, B. C. eds. The nude mouse in experimental and clinical research. New York: Academic Press; 1978: 187–214.Google Scholar
- 25.Reed, N. D.; Manning, D. D. Present status of xenotransplantation of nonmalignant tissue to the nude mouse. Fogh, J.; Giovanella, B. C. eds. The nude mouse in experimental and clinical research. New York: Academic Press, 1978: 167–185.Google Scholar
- 26.Fogh, J.; Bean, M. A.; Bruggen, J.; Fogh, H.; Fogh, J. M.; Hammar, S. P.; Kodora, Y.; Loveless, J. D.; Sorg, C.; Wright, W. C. Comparison of a human tumor cell line before and after growth in the nude mouse. Fogh, J.; Giovanella, B. C. eds. The nude mouse in experimental and clinical research. New York: Academic Press; 1978: 215–234.Google Scholar