Cell Biophysics

, Volume 5, Issue 2, pp 129–140 | Cite as

TMA-DPH: A suitable fluorescence polarization probe for specific plasma membrane fluidity studies in intact living cells

  • Jean-Georges Kuhry
  • Paul Fonteneau
  • Guy Duportail
  • Clarisse Maechling
  • Gilbert Laustriat


Fluorescence intensity measurements and fluorescence microscopy data showed that TMA-DPH (trimethylammonium diphenylhexatriene), a cationic derivative of the fluorescence polarization probe DPH, has a considerably different behavior in L929 cultured cells than does its parent molecule. In contrast to DPH, it incorporates very rapidly in the plasma membranes of the treated cells, and remains specifically localized on the cell surface for at least 25 min. It can therefore be recommended for specific plasma membrane fluidity measurements in whole living cells. No relevant information about the localization of the probes could be obtained by other techniques used in parallel, namely: subcellular fractionation and fluorescence inhibition by trinitrobenzene sulfonate (TNBS).

Index Entries

TMA-DPH, use in plasma membrane fluidity studies DPH, use in plasma membrane fluidity studies cells, fluorescence studies of plasma membrane, fluorescence studies of plasma membrane fluidity, fluorescence studies of specific probe, of membrane fluidity fluorescence polarization, with a TMA-DPH probe 


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  1. 1.
    Axerod, J., and Hirata, F. (1982),TIPS 3, 156.Google Scholar
  2. 2.
    Hirata, F., and Axelrod, J. (1978),Nature 275, 219.PubMedCrossRefGoogle Scholar
  3. 3.
    Dave, J. R., Knazek, R. A., and Liu, S. C. (1981),Biochem. Biophys. Res. Comm. 100, 45.PubMedCrossRefGoogle Scholar
  4. 4.
    Mitchell, R. H., and Kirk, C. J. (1981),TIPS 2, 86.Google Scholar
  5. 5.
    Berridge, M. J. (1980),TIPS 1, 419.Google Scholar
  6. 6.
    Lussan, C., and Faucon, J. F. (1974),Biochim. Biophys. Acta 345, 83.PubMedCrossRefGoogle Scholar
  7. 7.
    Berlin, R. D., and Fera, J. P. (1977),Proc. Natl. Acad. Sci. USA 74, 1072.PubMedCrossRefGoogle Scholar
  8. 8.
    Cossins, A. R. (1977),Biochim. Biophys. Acta 470, 395.PubMedCrossRefGoogle Scholar
  9. 9.
    Shinitzky, M., and Yuli, I. (1982),Chem. Phys. Lipids 30, 261.CrossRefGoogle Scholar
  10. 10.
    Johnson, S. M., and Nicolau, C. (1977),Biochem. Biophys. Res. Comm. 76, 869.PubMedCrossRefGoogle Scholar
  11. 11.
    Grunberger, D., Haimovitz, R., and Shinitzky, M. (1982),Biochim. Biophys. Acta 688, 764.PubMedCrossRefGoogle Scholar
  12. 12.
    Prendergast, F. G., Haugland, R. P., and Callahan, P. J. (1981),Biochemistry 20, 7333.PubMedCrossRefGoogle Scholar
  13. 13.
    Shinitzky, M., and Barenholz, Y. (1974),J. Biol. Chem. 249, 2652.PubMedGoogle Scholar
  14. 14.
    Cooper, R. A., Leslie, M. H., Fischkoff, S., Shinitzky, M., and Shattil, J. (1978),Biochemistry 17, 327.PubMedCrossRefGoogle Scholar
  15. 15.
    Pettegrew, J. W., Minshew, N. J., Nichols, J. S. (1982),Life Sciences 30, 1101.PubMedCrossRefGoogle Scholar
  16. 16.
    Laduron, P. M., Ilien, B., and Janssen, P. F. M. (1983),J. Neurochem. (to be published).Google Scholar
  17. 17.
    Cooberstein, S. J., and Lazarow, A. (1951),J. Biol. Chem. 189, 665.Google Scholar
  18. 18.
    Sottocasa, G. L., Kuylenstierna, B., Ernster, L., and Bergstrand, A. (1967),J. Cell. Biol. 32, 415.PubMedCrossRefGoogle Scholar
  19. 19.
    Brunette, D. M., and Till, J. E. (1971),J. Membr. Biol. 5, 215.CrossRefGoogle Scholar
  20. 20.
    Ipata, P. L. (1967),Anal. Biochem. 20, 30.PubMedCrossRefGoogle Scholar
  21. 21.
    Hubbard, A. L., and Cohn, Z. A. (1975),J. Cell Biol. 64, 438.PubMedCrossRefGoogle Scholar
  22. 22.
    Schacterle, G. R., and Pollack, R. L. (1973),Anal. Biochem. 51, 654.PubMedCrossRefGoogle Scholar
  23. 23.
    Okuyama, T., and Satake, K. (1960),J. Biochem. Tokyo 47, 454.Google Scholar
  24. 24.
    Förster, J. (1948),Ann. Physik. 2, 55.CrossRefGoogle Scholar
  25. 25.
    Duportail, G., and Weinreb, A. (1983),Biophys. Biochim. Acta. Google Scholar
  26. 26.
    Hubbard, A. L., and Cohn, Z. A. (1976), Specific Labels for Cell Surfaces, inBiochemical Analysis of Membranes (Maddy, A. H., ed.) Chapman, Hall, London, John Wiley, New York, p. 444.Google Scholar

Copyright information

© Humana Press Inc 1983

Authors and Affiliations

  • Jean-Georges Kuhry
    • 1
  • Paul Fonteneau
    • 2
  • Guy Duportail
    • 1
  • Clarisse Maechling
    • 1
  • Gilbert Laustriat
    • 1
  1. 1.Laboratorire de Physique, ERA 551 du CNRSUER des Sciences PharmaceutiquesStrasbourg CédexFrance
  2. 2.Laboratoire d'ImmunologieInstitut de Chimie BiologiqueStrasbourg Cédex

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