Abstract
Zinc participates extensively in the metabolism of carbohydrates, lipids, proteins, and nucleic acids and therefore is essential for the growth and development of all organs, including the brain. The concentrations of zinc in various regions of developing rat brain are nonuniform, and either remain the same or decline dramatically. Studies involving gel permeation chromatography on Sephadex G-75 have shown that unlike the hepatic metallothionein, the concentration of a metallothionein-like protein increases postnatally in the brain from 0.2 μg in 1 d after birth to 3.60 μg zinc/mg protein in 50 d after birth. Furthermore, high-performance liquid chromatographic studies have shown that the adult rat brain contains three small-molecular-weight zinc-binding proteins, one of which is stimulated following intracerebroventricular administration of zinc, producing metallothionein-like isoforms I and II, with retention times of 17.32 and 18.64 min, respectively. All three zinc-binding proteins are absent in the brains of newborn rats. It is proposed that the developmental alteration in the concentration of brain metallothionein-like protein may be related to zinc-mediated functions associated with the development and the maturation of brain.
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References
B. L. Vallee, inZinc Enzymes, vol. 5, T. G. Spiro, ed., John Wiley, New York, NY, 1983, pp. 3–24.
L. S. Hurley, J. Gowan, and H. Swenerton,Teratology 4, 199 (1971).
L. S. Hurley,Physiol. Rev. 61, 249 (1981).
M. Ebadi,J. Nutr. Growth Cancer 2, 81 (1985).
H. H. Sandstead, G. J. Fosmire, E. S. Halas, R. A. Jacob, D. S. Strobel, and E. O. Marks,Am. J. Clin. Nutr. 31, 844 (1977).
H. H. Sandstead, D. A. Strobel, G. M. Logan, E. O. Marks, and R. A. Jacob,Am. J. Clin. Nutr. 31, 844 (1978).
E. S. Halas and H. H. Sandstead,Pediatr. Res. 9, 94 (1975).
E. S. Halas, G. Reynolds, M. Rowe, M. Heinrich, and M. Pirc,Physiol. Behav. 18, 975 (1977).
D. P. Peters,Physiol. Behav. 20, 359 (1978).
E. S. Halas and H. H. Sandstead,Pediatr. Res. 9, 94 (1975).
E. S. Halas, M. D. Heinrich, and H. H. Sandstead,Physiol. Behav. 22, 991 (1979).
E. S. Halas, J. J. Eberhardt, M. A. Diers, and H. H. Sandstead,Physiol. Behav. 30, 371 (1983).
D. P. Peters,Physiol. Behav. 22, 1067 (1979).
M. S. Golub, M. E. Gershwin, and V. K. Vijayan,Physiol. Behav. 30, 409 (1983).
E. F. Gordon, inThe Neurobiology of Zinc. Part B: C. J. Frederickson, G. A. Howell, and E. J. Kasarskis, eds., Liss, New York, NY, 1984, pp. 77–98.
E. S. Halas and J. C. Kawamoto, inThe Neurobiology of Zinc. Part B: C. J. Frederickson, G. A. Howell, and E. J. Kasarskis, eds., Liss, New York, NY, 1984, pp. 91–107.
D. S. Olton, C. Collison, and M. Werz,Learn. Motivat. 8, 289 (1977).
D. S. Olton, J. A. Walker, and F. H. Gage,Brain Res. 139, 295 (1978).
C. L. Dvergsten, G. J. Fosmire, D. A. Ollerich, and H. H. Sandstead,Brain Res. 271, 217 (1983).
C. L. Dvergsten, G. J. Fosmire, D. A. Ollerich, and H. H. Sandstead,Dev. Brain Res. 16, 11 (1984).
C. L. Dvergsten, L. A. Johnson, and H. H. Sandstead,Dev. Brain Res. 16, 21 (1984).
R. Mason, A. Bakka, G. P. Samarawickrama, and M. Webb,Br. J. Nutr. 45, 375 (1980).
R. Mason, F. O. Brady, and M. Webb,Br. J. Nutr. 45, 391 (1981).
F. O. Brady and M. Webb,J. Biol. Chem. 256, 3931 (1981).
F. O. Brady,Life Sci. 32, 2981 (1983).
G. W. Evans, P. E. Johnson, J. G. Brushmiller, and R. W. Ames,Anal. Chem. 51, 839 (1979).
M. Itoh, M. Ebadi, and S. Swanson,J. Neurochem. 41, 823, (1983).
M. Ebadi, R. J. White, and S. Swanson, inThe Neurobiology of Zinc, Part A: C. J. Frederickson, G. A. Howell, and E. J. Karaskis, eds., Liss, New York, NY, 1984, pp. 39–57.
M. Ebadi and J. C. Wallwork,Biol. Trace Elem. Res. 7, 129 (1985).
S. Klauser, J. H. R. Kagi, and K. J. Wilson,Biochem. J. 209, 71 (1983).
K. Suzuki, N. Sunaga, and T. Yajima,J. Chromatog. 303, 131 (1984).
R. H. O. Buhler and J. H. R. Kagi,FEBS Lett. 39, 229 (1974).
M. Kimura, N. Otaki, and M. Imano, inMetallothioneins, J. H. R. Kagi and M. Nordberg, eds., tBirkhauser, Basel, (1979), pp. 163–168.
M. M. Bradford,Anal. Biochem. 72, 248 (1976).
F. R. Konig, and R. A. Klippel, inThe Rat Brain. William and Wilkins, Baltimore, MD 1963, p. 1.
H. Scheffe, inThe Analysis of Variance. Wiley, New York, NY, 1959, pp. 90–137.
J. Smeyers-Verbeke, E. Defrise-Gussenhoven, G. Ebinger, A. Lowenthal, and D. L. Massart,Clin. Chim. Acta 51, 309 (1974).
L. Wuyts, J. Smeyers-Verbeke, and D. L. Massart,Clin. Chim. Acta 72, 405 (1976).
I. L. Crawford and J. D. Connor,J. Neurochem. 19, 1451 (1972).
R. Kishi, T. Ikeda, H. Miyake, E. Uchino, T. Tsuzuki, and K. Inoue,Brain Res. 251, 180 (1982).
K. L. Wong and C. D. KlaassenToxicol. Appl. Pharmacol. 53, 343 (1980).
S. Jugo,Health Phys. 30, 204 (1976).
B. Momcilovic and K. Kostial,Environ. Res. 8, 214 (1974).
E. J. Underwood,Trace Elements in Human and Animal Nutrition, 4th Ed. Academic, New York, NY, 1977, pp. 56–108.
C. E. Casey, B. E. Guthrie, and M. F. Robinson,Biol. Trace Elem. Res. 4, 105 (1982).
G. W. Evans and E. C. Johnson,J. Nutr. 111, 68 (1981).
P. Blakeborough, D. N. Salter, and M. I. Gurr,Biochem. J. 209, 505 (1983).
S. M. Sato, J. M. Frazier, and A. M. Goldberg,J. Neurosci. 4, 1671 and 1552 (1984).
J. U. Bell,Toxicol. Appl. Pharmacol. 50, 101 (1979).
K. L. Wong and C. D. Klaassen,J. Biol. Chem. 254, 12399 (1979).
M. Panemangalore, D. Banarjee, S. Onosaka, and M. G. Cherian,Dev. Biol. 97, 95 (1983).
D. M. Templeton, D. Banerjee, and M. G. Cherian,Can. J. Biochem. Cell Biol. 63, 16 (1985).
L. Ryden and H. F. Deutsch,J. Biol. Chem. 253, 519 (1978).
J. R. Riordan and V. Richards,J. Biol. Chem. 255, 5380 (1980).
H. Porter,Biochem. Biophys. Res. Commun. 56, 661 (1974).
I. Bremner and R. K. Mehra,Chem. Sci. 21, 117 (1983).
S. H. Oh and P. D. Whanger,Am. J. Physiol. 237, E18 (1979).
J. W. Ridlington, D. E. Gorger, D. C. Chapman, and P. D. Whanger,Biol. Trace Elem. Res. 5, 175 (1983).
D. R. Winge, K. B. Nielson, R. D. Zeikus, and W. R. Gray,J. Biol. Chem. 259, 11419 (1984).
K. Munger, U. A. Germann, M. Beltramini, D. Niedermann, G. Baitella-Eberle, J. H. R. Kagi, and K. Lerch,J. Biol. 260, 10032 (1985).
M. Ebadi and Y. Hama, inExcitatory Amino Acids and Epilepsy, Y. Ben-Ari and R. Schwarcz, eds., Plenum, New York, NY, 1986, pp. 557–578.
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Ebadi, M. Biochemical alteration of a metallothionein-like protein in developing rat brain. Biol Trace Elem Res 11, 117–128 (1986). https://doi.org/10.1007/BF02795529
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DOI: https://doi.org/10.1007/BF02795529