The effect of copper ion on glutathione and hemolysis in rabbit erythrocytes
The rate of hemolysis and the decline in glutathione (GSH) in rabbit erythrocytes caused by copper (Cu) ions were determined. Prior investigations have proposed that the oxidative stress induced by Cu ion depleted the normal cell protective mechanisms. The decline in GSH has been proposed as a necessary prerequisite for hemolysis. We have observed that both GSH decline and hemolysis are Cu dependent, but are two concurrent and independent processes. We have confirmed that oxygen is a necessary reactant for hemolysis and responsible for a major portion of GSH decline. However, in the presence of Cu ion, a slow decline in GSH occurs even in a deaerated system.
Index EntriesHemolysis, in rabbit erythrocytes rabbit erythrocytes glutathione, effect of copper on copper dependence for hemolysis copper dependence for glutathione decline copper phosphate copper, ion, effect of on glutathione in rabbit erythrocytes copper ion, effect of on hemolysis in rabbit erythrocytes
Unable to display preview. Download preview PDF.
- 2.E. M. Crook,Glutathione-Biochemical Society Symposium #17,9, University Press, Cambridge, 1959.Google Scholar
- 3.T. Sivertsen,Acta Pharmacol. Toxicol. 46, 121 (1980).Google Scholar
- 4.A. A. Schilt,Analytical Applications of 1, 10-phenanthroline and Related Compounds, Pergamon, Oxford, 1969, p. 74.Google Scholar
- 5.E. Buetler,Red Cell Metabolism, Grune and Stratton, Inc., Third Ed., Orlando, Fl, 1984, p. 131.Google Scholar